用于检测 I-III 型干扰素及其抗体中和的高灵敏报告细胞系。

IF 4.5 3区医学 Q2 IMMUNOLOGY

European Journal of Immunology Pub Date : 2024-10-04 DOI:10.1002/eji.202451325

Kevin Groen, Roger Kuratli, Lauren Sar, Andri Vasou, Michael Huber, David J Hughes, Benjamin G Hale

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引用次数: 0

摘要

干扰素（IFNs）是先天性免疫防御系统的重要组成部分，能限制病毒性疾病的严重程度。为了推进对 IFNs 及其被致病性自身抗体中和的研究，我们生成了一种基于雷尼拉荧光素酶的报告细胞系，它能够检测 IFN-Is、IFN-II 和 IFN-IIIs 的活性。该报告细胞系对 IFNs 的灵敏度比常用的替代生物报告系统高出 125 到 2000 倍，并能以快速、简单的活细胞工作流程检测低滴度的中和抗 IFN 抗体。

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Highly sensitive reporter cell line for detection of interferon types I-III and their neutralization by antibodies.

Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.

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来源期刊

European Journal of Immunology 医学-免疫学

CiteScore

8.30

自引率

3.70%

发文量

224

审稿时长

2 months

期刊介绍： The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.