利用衣藻中的模块化克隆技术进行基因元件标准化组装的金门克隆。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4220-7_25

Peter Emelin, Sarah Abdul-Mawla, Felix Willmund

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引用次数: 0

摘要

现代合成生物学需要快速高效的克隆策略来组装新的转录单元或整个通路。模块化克隆（MoClo）是一种标准化的合成生物学工作流程，极大地简化了转基因表达遗传元件的组装。MoClo 以 Golden Gate Assembly 为基础，允许通过分层语法驱动的管道组合基因库中的遗传元件。在这里，我们描述了在单细胞模式藻类衣藻中组装转基因表达基因盒的过程。

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Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas.

Modern synthetic biology requires fast and efficient cloning strategies for the assembly of new transcription units or entire pathways. Modular Cloning (MoClo) is a standardized synthetic biology workflow, which has tremendously simplified the assembly of genetic elements for transgene expression. MoClo is based on Golden Gate Assembly and allows to combine genetic elements of a library through a hierarchical syntax-driven pipeline. Here we describe the assembly of a genetic cassette for transgene expression in the single-celled model alga Chlamydomonas reinhardtii.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.