Iana Aragão Magalhães, Gabriella Alves Julião Costa, André Alves Crispim, Thinali Sousa Dantas, Fabrício Bitu Sousa, Maria Júlia Barbosa Bezerra, Paulo Goberlânio de Barros Silva
{"title":"错配修复蛋白免疫染色对唾液腺腺样囊性癌临床病理特征和预后的影响","authors":"Iana Aragão Magalhães, Gabriella Alves Julião Costa, André Alves Crispim, Thinali Sousa Dantas, Fabrício Bitu Sousa, Maria Júlia Barbosa Bezerra, Paulo Goberlânio de Barros Silva","doi":"10.1111/jop.13585","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>To evaluate the influence of MMR proteins on clinicopathological characteristics and prognosis of salivary gland adenoid cystic carcinoma (ACC).</p>\n </section>\n \n <section>\n \n <h3> Method</h3>\n \n <p>The solid pattern of ACC showed lower expression for MSH2 (<i>p</i> = 0.039). Significant imbalance in MSH2/MSH6 immunostaining was observed in all histological patterns (<i>p</i> < 0.001), and imbalance in PMS2/MLH1 immunostaining was observed in the cribriform pattern (<i>p</i> = 0.011). The presence of capsule was associated with high expression of MSH6 (<i>p</i> = 0.019), MLH1 (<i>p</i> = 0.045) and PMS2 (<i>p</i> = 0.009). The absence of cribriform pattern (<i>p</i> = 0.002) and capsule pattern (<i>p</i> = 0.025), as well as low expression for MSH6 (<i>p</i> = 0.006) and PMS2 (<i>p</i> = 0.037) were associated with lower overall survival. In multivariate analysis, loss of MSH2 (<i>p</i> = 0.039) and MLH1 (<i>p</i> = 0.017) were significantly associated with worse overall survival.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Twenty-four ACC were clinical-pathologically evaluated and we perform immunohistochemistry for MSH2, MSH6, PMS2 and MLH1. Percentage counting of positive cells was performed in 10 fields of each histological pattern (cribriform, tubular and solid) and the averages of the 30 fields were considered for evaluation with other clinical-pathological variables (Kruskal-Wallis/Dunn, Friedman/Dunn, chi-square, Log-Rank Mantel-Cox tests and Cox regression; SPSS v20.0, <i>p</i> < 0.05).</p>\n </section>\n \n <section>\n \n <h3> Discussion</h3>\n \n <p>Salivary glands' ACC shows imbalance of the MMR complex and loss of expression of its components is associated with the overall survival of these patients.</p>\n </section>\n </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"53 10","pages":"637-647"},"PeriodicalIF":2.7000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunostaining for Mismatch Repair Complex Proteins Impacts on Clinical-Pathological Characteristics and Prognosis of Adenoid Cystic Carcinoma of Salivary Glands\",\"authors\":\"Iana Aragão Magalhães, Gabriella Alves Julião Costa, André Alves Crispim, Thinali Sousa Dantas, Fabrício Bitu Sousa, Maria Júlia Barbosa Bezerra, Paulo Goberlânio de Barros Silva\",\"doi\":\"10.1111/jop.13585\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To evaluate the influence of MMR proteins on clinicopathological characteristics and prognosis of salivary gland adenoid cystic carcinoma (ACC).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Method</h3>\\n \\n <p>The solid pattern of ACC showed lower expression for MSH2 (<i>p</i> = 0.039). Significant imbalance in MSH2/MSH6 immunostaining was observed in all histological patterns (<i>p</i> < 0.001), and imbalance in PMS2/MLH1 immunostaining was observed in the cribriform pattern (<i>p</i> = 0.011). The presence of capsule was associated with high expression of MSH6 (<i>p</i> = 0.019), MLH1 (<i>p</i> = 0.045) and PMS2 (<i>p</i> = 0.009). The absence of cribriform pattern (<i>p</i> = 0.002) and capsule pattern (<i>p</i> = 0.025), as well as low expression for MSH6 (<i>p</i> = 0.006) and PMS2 (<i>p</i> = 0.037) were associated with lower overall survival. In multivariate analysis, loss of MSH2 (<i>p</i> = 0.039) and MLH1 (<i>p</i> = 0.017) were significantly associated with worse overall survival.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Twenty-four ACC were clinical-pathologically evaluated and we perform immunohistochemistry for MSH2, MSH6, PMS2 and MLH1. 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Immunostaining for Mismatch Repair Complex Proteins Impacts on Clinical-Pathological Characteristics and Prognosis of Adenoid Cystic Carcinoma of Salivary Glands
Objective
To evaluate the influence of MMR proteins on clinicopathological characteristics and prognosis of salivary gland adenoid cystic carcinoma (ACC).
Method
The solid pattern of ACC showed lower expression for MSH2 (p = 0.039). Significant imbalance in MSH2/MSH6 immunostaining was observed in all histological patterns (p < 0.001), and imbalance in PMS2/MLH1 immunostaining was observed in the cribriform pattern (p = 0.011). The presence of capsule was associated with high expression of MSH6 (p = 0.019), MLH1 (p = 0.045) and PMS2 (p = 0.009). The absence of cribriform pattern (p = 0.002) and capsule pattern (p = 0.025), as well as low expression for MSH6 (p = 0.006) and PMS2 (p = 0.037) were associated with lower overall survival. In multivariate analysis, loss of MSH2 (p = 0.039) and MLH1 (p = 0.017) were significantly associated with worse overall survival.
Results
Twenty-four ACC were clinical-pathologically evaluated and we perform immunohistochemistry for MSH2, MSH6, PMS2 and MLH1. Percentage counting of positive cells was performed in 10 fields of each histological pattern (cribriform, tubular and solid) and the averages of the 30 fields were considered for evaluation with other clinical-pathological variables (Kruskal-Wallis/Dunn, Friedman/Dunn, chi-square, Log-Rank Mantel-Cox tests and Cox regression; SPSS v20.0, p < 0.05).
Discussion
Salivary glands' ACC shows imbalance of the MMR complex and loss of expression of its components is associated with the overall survival of these patients.
期刊介绍：
The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.
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