Andreas Kontny, Dimo Stoyanov, Pavel Pavlov, Neele Wagner, Nikola Kolev, Alexander Zlatarov, Turgay Kalinov, Anton B Tonchev
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On-slide clearing and imaging of 100-µm-thick histological sections using ethyl cinnamate and epifluorescence.
Introduction: Thick histological samples are difficult to image without proper tissue clearing methods. Among these methods ethyl cinnamate (ECi)-based clearing preserves antigenicity and is compatible with immunofluorescent labeling. In contrast to many other clearing protocols, ECi-based clearing is fast and is done as a final step after standard immunofluorescent labeling protocols.
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