快速简便的改良硝酸还原酶测定法，用于检测结核分枝杆菌分离株对一线和二线抗结核药物的敏感性。

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-09-26 DOI:10.1016/j.diagmicrobio.2024.116547

Ece Simsek , Kubra Yildirim , Cemilenur Atas , Ahmet Yilmaz Coban

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引用次数: 0

摘要

在这项研究中，我们开发了一种改良的 NRA（MONRA），用于测定 5 株参考 ATCC 菌株和 42 株临床结核杆菌分离株的一线和二线药物敏感性。与通常在固体培养基或 7H9 肉汤中进行的传统 NRA 不同，MONRA 是在不同的培养基 AYC.2.1 肉汤中进行的，使用冻干抗生素试管测定药物敏感性。对于一线药物，MONRA 结果与 BACTEC MGIT 960 方法（参考方法）进行了比较；对于二线药物，则与在 7H9 肉汤中进行的传统 NRA 方法进行了比较。经测定，链霉素、异烟肼、利福平和乙胺丁醇的MONRA结果与参考方法的一致性分别为97.62%、100%、97.62%和100%。当将结果与 convantional NRA 进行比较时，所有二线抗生素（包括左氧氟沙星、氧氟沙星和卡那霉素）的一致性被确定为 100%。MONRA有可能消除在资源有限的环境中实施药敏试验所面临的挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A rapid and simple modified nitrate reductase assay for testing first and second-line antituberculosis drug susceptibilities in Mycobacterium tuberculosis isolates

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A rapid and simple modified nitrate reductase assay for testing first and second-line antituberculosis drug susceptibilities in Mycobacterium tuberculosis isolates

In this study, we developed a modified NRA (MONRA) to determine the first and second-line drug susceptibilities of 5 reference ATCC strains and 42 clinical M. tuberculosis isolates. Unlike conventional NRA, which is often performed in solid media or 7H9 broth, the MONRA is performed in a different medium, AYC.2.1 broth, using lyophilized antibiotic tubes to determine drug susceptibility. The MONRA results were compared with BACTEC MGIT 960 method as the reference method for first-line drugs and conventional NRA performed in 7H9 broth for second-line drugs. The agreement between the MONRA and the reference method was determined as 97.62, 100, 97.62, and 100 % for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. When the results were compared with convantional NRA, the agreement was determined as 100 % for all second-line antibiotics including levofloxacin, ofloxacin, and kanamycin. MONRA has the potential to eliminate challenges in implementing drug susceptibility testing in resource-limited settings.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.