使用微容器进行模拟开放式采集时,血容量、空气暴露持续时间、运输持续时间和测试延迟对血浆总二氧化碳的影响。

IF 2.5 3区 医学 Q2 MEDICAL LABORATORY TECHNOLOGY
Michael S. Reid , Isolde Seiden Long , Lawrence de Koning
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引用次数: 0

摘要

背景:将血液标本暴露在空气中会降低血浆总二氧化碳(TCO2)。我们评估了在 BD 微容器®(微容器)中模拟开放式采集新生儿血液的 TCO2 降低程度、微容器运输持续时间以及开放式血浆等分的延迟测试:采用 3x4 因子设计将静脉血等分到开放式微容器中,以模拟血容量(0.2-0.6 毫升)和空气暴露持续时间(0-5 分钟)的综合影响,并以空泡采血器抽取的血液作为对照。通过重复试验分别评估了院内运送时间(0-120 分钟;全血)、院外运送时间(0-24 小时;离心全血)和血浆等分开放时间(0-120 分钟)的影响。结果采用重复测量方差分析和学生 T 检验进行分析:结果:在因子实验中,所有微量容器中的平均血浆 TCO2 平均比真空容器中低 3.5 mmol/L。较小的血容量与较长的空气暴露时间相比有显著差异(p 2.0)。灌装(0.6 毫升;1-5 分钟空气暴露)微量容器中的平均 TCO2 平均比真空容器中低 2.9 毫摩尔/升。对装有离心全血的微量容器进行模拟异地运输可显著降低 TCO2(4 小时;平均-1.5 毫摩尔/升),对等分血浆进行延迟测试也是如此(15 分钟;平均-1.3 毫摩尔/升):结论:血浆 TCO2 会随着微容器血容量的减少、离心血液运输时间的延长和等分血浆检测的延迟而降低。为尽量减少 TCO2 的降低,微量容器应完全灌满并快速检测。实验室还应考虑这些检测是否适合使用解释性注释、校正因子或单独的参考区间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of blood volume, air exposure duration, transport duration, and testing delay on plasma total carbon dioxide in simulated open collections using microtainers

Background

Exposing blood specimens to air reduces plasma total carbon dioxide (TCO2). We evaluated the degree of TCO2 reduction attributed to open collection of neonatal blood in BD microtainers® (microtainers), microtainer transport duration and delayed testing of open plasma aliquots.

Methods

Venous blood was aliquoted into open microtainers in a 3x4 factorial design to simulate combined effects of blood volume (0.2–0.6 mL) and air exposure duration (0–5 min), with blood drawn in vacutainers as a control. Separate effects of in-hospital transport duration (0–120 min; whole blood), off-site transport duration (0–24 h; centrifuged whole blood), and the duration plasma aliquots remained open (0–120 min) were evaluated by repeated testing. Findings were analyzed using repeated-measures ANOVA and Student’s T-tests.

Results

In the factorial experiment, mean plasma TCO2 in microtainers was on average 3.5 mmol/L lower than in vacutainers. Smaller blood volume but not greater air exposure duration significantly (p < 0.05) reduced TCO2. Mean TCO2 in filled (0.6 mL; 1–5 min air exposure) microtainers was on average 2.9 mmol/L lower than in vacutainers. Simulated off-site transport of microtainers containing centrifuged whole blood significantly reduced TCO2 (4 h; mean change = -1.5 mmol/L), as did delayed testing of aliquoted plasma (15 min; mean change = -1.3 mmol/L).

Conclusions

Plasma TCO2 decreased with reduced microtainer blood volume, extended off-site transport duration of centrifuged whole blood and testing delay of aliquoted plasma. To minimize TCO2 reduction, microtainers should be fully filled and tested rapidly. Laboratories should also consider whether an interpretive comment, correction factor or separate reference intervals are appropriate for these tests.
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来源期刊
Clinical biochemistry
Clinical biochemistry 医学-医学实验技术
CiteScore
5.10
自引率
0.00%
发文量
151
审稿时长
25 days
期刊介绍: Clinical Biochemistry publishes articles relating to clinical chemistry, molecular biology and genetics, therapeutic drug monitoring and toxicology, laboratory immunology and laboratory medicine in general, with the focus on analytical and clinical investigation of laboratory tests in humans used for diagnosis, prognosis, treatment and therapy, and monitoring of disease.
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