显微凝集试验是诊断牛生殖器钩端螺旋体病综合征的可靠方法吗?

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES
Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-28 DOI:10.1007/s11259-024-10560-4
Luiza Aymée, Ana Luiza Dos Santos Baptista Borges, Guilherme Nunes de Souza, Walter Lilenbaum
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引用次数: 0

摘要

牛生殖器钩端螺旋体病(BGL)是一种以生殖器感染钩端螺旋体为特征的慢性繁殖综合征,准确诊断BGL对在田间条件下实施适当的控制措施至关重要。本研究旨在评估显微镜凝集试验(MAT)血清学诊断生殖器感染的亚发情奶牛钩端螺旋体病的可靠性。在三个牛群中,93 头繁殖失败的非怀孕奶牛接受了血液采样(MAT 血清学检测)和生殖器采样(lipL32-PCR)。62/93(66.6%)头奶牛的血清反应呈100分界线阳性,45/93(48.4%)头奶牛的血清反应呈200分界线阳性,主要是针对Sejroe血清群。在 PCR 分析中,55/93(59.1%)头奶牛呈阳性。将 MAT 结果与 PCR(标准)进行了比较,并计算了 100 和 200 临界值的检测参数和 Cohen's kappa (ƙ)。对滴度 100 至 1,600 的每个临界值都绘制了 ROC 曲线。计算得出,MAT100 的灵敏度和特异性分别为 66.6% 和 33.3%,而 MAT200 的灵敏度估计为 35%,特异性为 54.5%。MAT 的准确性较差,MAT100 为 54.8%,MAT200 为 42%。此外,所有滴度的 ROC 分析曲线下面积均较低,MAT100 和 MAT200 的相关性较差(ƙ < 0)。结果表明,MAT 是一种用于单独诊断牛生殖器带菌者的有限技术,如果仅应用 MAT,生殖器带菌者可能会被漏检,从而影响控制计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Is microscopic agglutination test a reliable method for diagnosing the bovine genital leptospirosis syndrome?

Bovine Genital Leptospirosis (BGL) is a chronic reproductive syndrome characterized by genital infection by Leptospira spp. An accurate diagnosis of BGL is crucial to implementing proper control measures in field conditions. This study aimed to evaluate the reliability of serology by Microscopic Agglutination Test (MAT) for diagnosing leptospirosis in subfertile cows with genital infection. Of three herds, 93 non-pregnant cows with reproductive failures were submitted to the blood sampling (serology by MAT) and genital samples (lipL32-PCR). A total of 62/93 (66.6%) cows presented seroreactive to cutoff 100, while 45/93 (48.4%) cows were positive to cutoff 200, mainly against the Sejroe serogroup. In PCR analysis, 55/93 (59.1%) were positive. MAT results were compared with PCR (considered the standard), and test parameters and Cohen's kappa (ƙ) were calculated for the cut-offs 100 and 200. A ROC curve was performed for each cut-off of titers 100 to 1,600. The sensitivity and specificity of MAT100 were calculated at 66.6% and 33.3%, while for MAT200 the sensitivity was estimated as 35% and specificity as 54.5%. The accuracy of MAT was poor, being 54.8% in MAT100 and 42% in MAT200. Furthermore, the area under the curve of ROC analysis was low for all titers, and the correlation was poor for MAT100 and MAT200 (ƙ < 0). The results demonstrated that MAT is a limited technique to diagnose bovine genital carriers individually, and if only MAT is applied, genital carriers may pass undetected, impairing the control programs.

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来源期刊
Veterinary Research Communications
Veterinary Research Communications 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
173
审稿时长
3 months
期刊介绍: Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
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