Yang Han, Zeng Wang, Bing Han, Yingjun Zhang, Jindong Liu, Yan Yang
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Based on two SNPs between the sequences of TaABI5-A4a and TaABI5-A4b, two complementary dominant sequence-tagged site (STS) markers were developed and validated in a natural population of 103 Chinese wheat cultivars and advanced lines and 200 recombinant inbred lines (RILs) derived from the Yangxiaomai/Zhongyou 9507 cross; the STS markers can be used efficiently and reliably to evaluate the dormancy of wheat seeds. The transcription level of TaABI5-A4b was significantly increased in TaABI5-A4a-GFP transgenic rice lines compared with that in TaABI5-A4b-GFP. The average seed germination index of TaABI5-A4a-GFP transgenic rice lines was significantly lower than those of TaABI5-A4b-GFP. In addition, seeds of TaABI5-A4a-GFP transgenic lines had higher ABA sensitivity and endogenous ABA content, lower endogenous GA content and plant height, and thicker stem internodes than those of TaABI5-A4b-GFP. Allelic variation of TaABI5-A4-affected wheat seed dormancy and the gene function was confirmed in transgenic rice. The transgenic rice lines of TaABI5-A4a and TaABI5-A4b had significantly different sensitivities to ABA and contents of endogenous ABA and GA in mature seeds, thereby influencing the seed dormancy, plant height, and stem internode length and diameter.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":null,"pages":null},"PeriodicalIF":4.4000,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Allelic variation of TaABI5-A4 significantly affects seed dormancy in bread wheat.\",\"authors\":\"Yang Han, Zeng Wang, Bing Han, Yingjun Zhang, Jindong Liu, Yan Yang\",\"doi\":\"10.1007/s00122-024-04753-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Key message: </strong>We identified a pivotal transcription factor TaABI5-A4 that is significantly associated with pre-harvest sprouting in wheat; its function in regulating seed dormancy was confirmed in transgenic rice. 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引用次数: 0
摘要
关键信息:我们发现了一个关键的转录因子TaABI5-A4,它与小麦收获前萌发显著相关;在转基因水稻中证实了它在调控种子休眠方面的功能。ABI5 是调控农作物种子成熟、休眠、萌发和萌发后的关键转录因子。在中国小麦品系周8425B中发现了16个拷贝的ABI5同源序列。两个单倍型 TaABI5-A4a 和 TaABI5-A4b 的品种表现出显著不同的种子休眠。基于TaABI5-A4a和TaABI5-A4b序列之间的两个SNPs,开发了两个互补的显性序列标记位点(STS)标记,并在103个中国小麦栽培品种和先进品系以及200个杨小麦/中优9507杂交重组近交系(RIL)的自然群体中进行了验证;STS标记可用于高效、可靠地评价小麦种子休眠。与TaABI5-A4b-GFP相比,TaABI5-A4a-GFP转基因水稻株系中TaABI5-A4b的转录水平显著提高。TaABI5-A4a-GFP 转基因水稻株系的平均种子发芽指数明显低于 TaABI5-A4b-GFP。此外,与 TaABI5-A4b-GFP 相比,TaABI5-A4a-GFP 转基因品系种子的 ABA 敏感性和内源 ABA 含量更高,内源 GA 含量和株高更低,茎节间更粗。TaABI5-A4 影响小麦种子休眠的等位基因变异及其基因功能在转基因水稻中得到了证实。TaABI5-A4a和TaABI5-A4b的转基因水稻品系对ABA的敏感性以及成熟种子中内源ABA和GA的含量有显著差异,从而影响了种子休眠、株高、茎节间长度和直径。
Allelic variation of TaABI5-A4 significantly affects seed dormancy in bread wheat.
Key message: We identified a pivotal transcription factor TaABI5-A4 that is significantly associated with pre-harvest sprouting in wheat; its function in regulating seed dormancy was confirmed in transgenic rice. ABI5 is a critical transcription factor in regulation of crop seed maturation, dormancy, germination, and post-germination. Sixteen copies of homologous sequences of ABI5 were identified in Chinese wheat line Zhou 8425B. Cultivars of two haplotypes TaABI5-A4a and TaABI5-A4b showed significantly different seed dormancies. Based on two SNPs between the sequences of TaABI5-A4a and TaABI5-A4b, two complementary dominant sequence-tagged site (STS) markers were developed and validated in a natural population of 103 Chinese wheat cultivars and advanced lines and 200 recombinant inbred lines (RILs) derived from the Yangxiaomai/Zhongyou 9507 cross; the STS markers can be used efficiently and reliably to evaluate the dormancy of wheat seeds. The transcription level of TaABI5-A4b was significantly increased in TaABI5-A4a-GFP transgenic rice lines compared with that in TaABI5-A4b-GFP. The average seed germination index of TaABI5-A4a-GFP transgenic rice lines was significantly lower than those of TaABI5-A4b-GFP. In addition, seeds of TaABI5-A4a-GFP transgenic lines had higher ABA sensitivity and endogenous ABA content, lower endogenous GA content and plant height, and thicker stem internodes than those of TaABI5-A4b-GFP. Allelic variation of TaABI5-A4-affected wheat seed dormancy and the gene function was confirmed in transgenic rice. The transgenic rice lines of TaABI5-A4a and TaABI5-A4b had significantly different sensitivities to ABA and contents of endogenous ABA and GA in mature seeds, thereby influencing the seed dormancy, plant height, and stem internode length and diameter.
期刊介绍:
Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.