外泌体 miR-502-5p 通过 Wnt/β-catenin 通路抑制血管生成,从而抑制胃癌的进展。

IF 1.7 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Irish Journal of Medical Science Pub Date : 2024-12-01 Epub Date: 2024-09-26 DOI:10.1007/s11845-024-03789-0
Yanwu Zhou, Rong Li
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引用次数: 0

摘要

背景:胃癌(GC)是全球关注的重大健康问题,是第五大常见癌症,也是癌症相关死亡的第三大原因。miR-502-5p在多种癌症中的作用已被研究,但其通过外泌体对胃癌的具体影响还不甚了解。本研究旨在探讨外泌体衍生的 miR-502-5p 在胃癌中的作用和机制:方法:采用 qRT-PCR 方法测定 miR-502-5p 在胃癌患者组织或血清中的差异表达。通过体外和体内实验评估了 miR-502-5p 对细胞增殖、迁移和侵袭的影响。还通过体内和体外实验探讨了外泌体-miR-502-5p抑制转移能力的潜力。此外,还利用免疫印迹法研究了 miR-502-5p 在胃癌中的作用机制:结果发现,miR-502-5p 能抑制胃癌细胞的增殖、迁移和侵袭。外泌体-miR-502-5p的表达与转移能力呈负相关,在体外和体内均表现出抑制转移的作用。此外,miR-502-5p 似乎还能通过 Wnt/β-catenin 通路抑制胃癌的血管生成:结论:miR-502-5p外泌体在胃癌的发生和发展过程中起抑制作用,这表明它有可能成为抗癌治疗的靶点或诊断生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosomal miR-502-5p suppresses the progression of gastric cancer by repressing angiogenesis through the Wnt/β-catenin pathway.

Background: Gastric cancer (GC) is a significant global health concern, ranking as the fifth most common cancer and the third leading cause of cancer-related deaths. The role of miR-502-5p in various cancers has been studied, but its specific impact on gastric cancer through exosomes is not well understood. This study aimed to investigate the role and mechanism of exosome-derived miR-502-5p in gastric cancer.

Methods: Differential expression of miR-502-5p in tissues or serum of GC patients was determined using qRT-PCR. The impact of miR-502-5p on cell proliferation, migration, and invasion was assessed through in vitro and in vivo experiments. The potential of exosome-miR-502-5p to inhibit metastatic ability was also explored by using vivo and vitro assay. Furthermore, the underlying mechanism of miR-502-5p in gastric cancer was investigated using western blotting.

Results: It was found that miR-502-5p suppressed the proliferation, migration, and invasion of gastric cancer cells. Exosome-miR-502-5p expression was negatively linked to metastatic ability and demonstrated inhibition of metastasis in vitro and in vivo. Additionally, miR-502-5p appeared to inhibit angiogenesis through the Wnt/β-catenin pathway in gastric cancer.

Conclusions: Exosomal miR-502-5p acts as a suppressor in the development and progression of gastric cancer, suggesting its potential as a target for anti-cancer therapy or as a diagnostic biomarker.

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来源期刊
Irish Journal of Medical Science
Irish Journal of Medical Science 医学-医学:内科
CiteScore
3.70
自引率
4.80%
发文量
357
审稿时长
4-8 weeks
期刊介绍: The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker. The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.
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