通过在大肠杆菌中表达的甲羟戊酸途径中添加固定化酶来提高异戊二烯的产量。

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-09-27 DOI:10.1007/s00449-024-03093-2

Shenghu Hao, Mei Xu, Lu Li, Luyao Wang, Zhongliang Su

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引用次数: 0

摘要

异戊二烯是橡胶生产中的重要成分，可利用大肠杆菌甲羟戊酸（MVA）途径生产，该方法具有绿色环保和可持续发展的优点。然而，由于中间产物积累过多，细胞生长受到抑制，酶活性逐渐降低，异戊二烯的产量难以提高。本研究将固定化酶加入到甲羟戊酸代谢菌（PT-P）生产异戊二烯的发酵过程中，探讨其对异戊二烯合成的影响。在最佳条件下，与单独进行 PT-P 发酵相比，该酶催化 MVA 的转化效率高达 50.86%，异戊二烯的产量增加了约 30%，达到 234.47 mg/L。

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Enhancing isoprene production by supplementing mevalonate pathway expressed in E. coli with immobilized enzymes.

Isoprene is an important component in rubber production, which can be produced using the E. coli mevalonic acid (MVA) pathway, and this method has the advantage of green environmental protection and sustainable. However, due to the excessive accumulation of intermediates, the growth of cells was inhibited and the enzyme activity decreased gradually, so it was difficult to increase the yield of isoprene. The immobilized enzyme has the characteristics of high stability and strong reusability, so in this study, the immobilized enzyme was added to the fermentation process of isoprene production by mevalonate metabolizing bacteria (PT-P), to explore the effect on isoprene synthesis. Under the optimum conditions, compared with PT-P fermentation alone, the enzyme catalyzes the conversion of MVA with an efficiency of up to 50.86%, and the yield of isoprene increased by about 30%, reaching 234.47 mg/L.

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.