抗坏血酸 2-葡萄糖苷可提高冷冻解冻的 C57Bl/6J 和 C57Bl/6N 小鼠精子的存活率、质量和生育能力。

IF 3.2 2区 医学 Q1 ANDROLOGY
Andrology Pub Date : 2024-09-27 DOI:10.1111/andr.13768
Marcello Raspa, Renata Paoletti, Ferdinando Scavizzi
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引用次数: 0

摘要

背景:抗坏血酸 2-葡萄糖苷(AA2G)是抗坏血酸的一种稳定形式,也是一种有效的抗氧化剂。抗坏血酸存在于睾丸和附睾中,有助于维持生殖器官的生理完整性。抗坏血酸的特性已被用于保护不同物种的精子免受氧化应激:冷冻 C57Bl/6J 和 C57Bl/6N 株系的精子,解冻后在有甲基-β-环糊精(MBCD)或没有甲基-β-环糊精(MBCD)的情况下,在获能培养基中添加 AA2G 进行分析。处理效果由 SCA 系统(Microptic)进行评估,分析速度、活力、形态和 DNA 片段。我们还检测了经处理精子的获能能力(CTC)、顶体反应(Coomassie Brillant Blue)和受精能力(体外受精):结果:AA2G 提高了精子质量和受精率,尤其是在与中性粒细胞生长因子(MBCD)联合使用时。我们观察到精子的运动能力、速度和活力明显提高,获能能力和顶体反应增强。这些改善显著提高了体外受精的成功率。获得的胚胎经培养后通常可达到囊胚期:本研究旨在确定 AA2G 能否在冷冻保存过程中保护小鼠精子。我们发现 AA2G 有保护精子的作用,它能提高精子的存活率,从而提高受精率:结论:这一新近改进的方案显示出重新激活冷冻保存在小鼠生物库和国际储存库(如欧洲小鼠突变体档案库(EMMA))中的 GEMMs 的潜力。这可以作为实现 3R 任务(替换、减少和改进)的关键工具,促进符合伦理和人道的研究实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa.

Background: Ascorbic acid 2-glucoside (AA2G) is a stabilized form of ascorbic acid and a potent antioxidant. Ascorbic acid is present in the testes and epididymis and helps maintain the physiological integrity of reproductive organs. Its properties have been utilized to protect spermatozoa of different species from oxidative stress.

Materials and methods: Spermatozoa of C57Bl/6J and C57Bl/6N strains were frozen and analyzed, after thawing, by supplementing the capacitation medium with AA2G, both in the presence and absence of methyl-β-cyclodextrin (MBCD). The effect of treatment was evaluated by SCA System (Microptic) analyzing the velocity, vitality, morphology, and the DNA fragmentation. We also examined sperm capacitation (CTC), acrosome reaction (Coomassie Brillant Blue), and fertility (in vitro fertilization) of treated spermatozoa.

Results: AA2G improved sperm quality and fertility particularly in association with MBCD. We observed a significant increase of sperm motility, velocity, and vitality associated with an enhanced capacitation and acrosome reaction. These improvements resulted in a marked increase in in vitro fertilization success. Embryos obtained were cultured and reached normally the blastocyst stage.

Discussion: This study aimed to determine if AA2G could safeguard mouse spermatozoa during cryopreservation. We found a protective effect of AA2G that increased sperm survivability resulting in higher fertilization rate.

Conclusion: This newly improved protocol shows potential for reanimating cryopreserved GEMMs stored in mouse biobanks and international repositories, such as the European Mouse Mutant Archive (EMMA). This can serve as a pivotal tool in fulfilling the 3Rs mission (replacement, reduction, and refinement), promoting ethical and humane research practices.

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来源期刊
Andrology
Andrology ANDROLOGY-
CiteScore
9.10
自引率
6.70%
发文量
200
期刊介绍: Andrology is the study of the male reproductive system and other male gender related health issues. Andrology deals with basic and clinical aspects of the male reproductive system (gonads, endocrine and accessory organs) in all species, including the diagnosis and treatment of medical problems associated with sexual development, infertility, sexual dysfunction, sex hormone action and other urological problems. In medicine, Andrology as a specialty is a recent development, as it had previously been considered a subspecialty of urology or endocrinology
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