{"title":"RCK1 基因对酿酒酵母耐氧化性的调控","authors":"","doi":"10.1016/j.freeradbiomed.2024.09.040","DOIUrl":null,"url":null,"abstract":"<div><div>Our previous work indicated that the quorum sensing (QS) effect could regulate the oxidative tolerance of <em>Saccharomyces cerevisiae</em>, and QS may impact oxidative and antioxidative metabolisms of <em>S. cerevisiae</em> by regulating the <em>RCK1</em> gene. Therefore, this work proposed a reasonable logic that <em>RCK1</em> could play roles in regulating the oxidative and antioxidative metabolisms of yeast cells. The results presented here suggested that the overexpression of <em>RCK1</em> has a regulatory effect on the reduction of ROS level and the promotion of oxidative tolerance of <em>S. cerevisiae</em>. The overexpression of <em>RCK1</em> promoted the ROS generation through activating the MAPK pathway; on the other hand, <em>RCK1</em>-regulated antioxidative metabolism played a more significant role to realize lower ROS level and higher oxidative tolerance of S288c-<em>RCK1</em> and <em>ΔARO80</em>-<em>RCK1</em> strains. To improve the fermentation performance of yeast while circumventing metabolic burden, a recombinant strain with over time-controlled overexpression of the <em>RCK1</em> gene (<em>i.e.</em>, S288c′-<em>RCK1</em> strain) derived from S288c strain was successfully constructed to achieve artificial regulation of yeast oxidative tolerance. Transcriptomics analysis was further performed on both <em>S. cerevisiae</em> wild-type and S288c′-<em>RCK1</em> strains to identify differentially expressed genes and analyze their functional pathway classification. This work is instructive for artificially modulating the oxidative tolerance of strains to enhance the fermentation performance of yeast.</div></div>","PeriodicalId":12407,"journal":{"name":"Free Radical Biology and Medicine","volume":null,"pages":null},"PeriodicalIF":7.1000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Regulation of the RCK1 gene on the oxidative tolerance of Saccharomyces cerevisiae\",\"authors\":\"\",\"doi\":\"10.1016/j.freeradbiomed.2024.09.040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Our previous work indicated that the quorum sensing (QS) effect could regulate the oxidative tolerance of <em>Saccharomyces cerevisiae</em>, and QS may impact oxidative and antioxidative metabolisms of <em>S. cerevisiae</em> by regulating the <em>RCK1</em> gene. Therefore, this work proposed a reasonable logic that <em>RCK1</em> could play roles in regulating the oxidative and antioxidative metabolisms of yeast cells. The results presented here suggested that the overexpression of <em>RCK1</em> has a regulatory effect on the reduction of ROS level and the promotion of oxidative tolerance of <em>S. cerevisiae</em>. The overexpression of <em>RCK1</em> promoted the ROS generation through activating the MAPK pathway; on the other hand, <em>RCK1</em>-regulated antioxidative metabolism played a more significant role to realize lower ROS level and higher oxidative tolerance of S288c-<em>RCK1</em> and <em>ΔARO80</em>-<em>RCK1</em> strains. To improve the fermentation performance of yeast while circumventing metabolic burden, a recombinant strain with over time-controlled overexpression of the <em>RCK1</em> gene (<em>i.e.</em>, S288c′-<em>RCK1</em> strain) derived from S288c strain was successfully constructed to achieve artificial regulation of yeast oxidative tolerance. Transcriptomics analysis was further performed on both <em>S. cerevisiae</em> wild-type and S288c′-<em>RCK1</em> strains to identify differentially expressed genes and analyze their functional pathway classification. This work is instructive for artificially modulating the oxidative tolerance of strains to enhance the fermentation performance of yeast.</div></div>\",\"PeriodicalId\":12407,\"journal\":{\"name\":\"Free Radical Biology and Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.1000,\"publicationDate\":\"2024-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Free Radical Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0891584924006877\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Free Radical Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0891584924006877","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Regulation of the RCK1 gene on the oxidative tolerance of Saccharomyces cerevisiae
Our previous work indicated that the quorum sensing (QS) effect could regulate the oxidative tolerance of Saccharomyces cerevisiae, and QS may impact oxidative and antioxidative metabolisms of S. cerevisiae by regulating the RCK1 gene. Therefore, this work proposed a reasonable logic that RCK1 could play roles in regulating the oxidative and antioxidative metabolisms of yeast cells. The results presented here suggested that the overexpression of RCK1 has a regulatory effect on the reduction of ROS level and the promotion of oxidative tolerance of S. cerevisiae. The overexpression of RCK1 promoted the ROS generation through activating the MAPK pathway; on the other hand, RCK1-regulated antioxidative metabolism played a more significant role to realize lower ROS level and higher oxidative tolerance of S288c-RCK1 and ΔARO80-RCK1 strains. To improve the fermentation performance of yeast while circumventing metabolic burden, a recombinant strain with over time-controlled overexpression of the RCK1 gene (i.e., S288c′-RCK1 strain) derived from S288c strain was successfully constructed to achieve artificial regulation of yeast oxidative tolerance. Transcriptomics analysis was further performed on both S. cerevisiae wild-type and S288c′-RCK1 strains to identify differentially expressed genes and analyze their functional pathway classification. This work is instructive for artificially modulating the oxidative tolerance of strains to enhance the fermentation performance of yeast.
期刊介绍:
Free Radical Biology and Medicine is a leading journal in the field of redox biology, which is the study of the role of reactive oxygen species (ROS) and other oxidizing agents in biological systems. The journal serves as a premier forum for publishing innovative and groundbreaking research that explores the redox biology of health and disease, covering a wide range of topics and disciplines. Free Radical Biology and Medicine also commissions Special Issues that highlight recent advances in both basic and clinical research, with a particular emphasis on the mechanisms underlying altered metabolism and redox signaling. These Special Issues aim to provide a focused platform for the latest research in the field, fostering collaboration and knowledge exchange among researchers and clinicians.