{"title":"多组学结合表达模式分析揭示马铃薯茉莉酸信号通路中关键基因对镉胁迫的调控响应","authors":"Mingfang Yang, Sanwei Yang, Weidong Wang, Xiaoliao Wei, Fei Lou, GuanDi He, Tengbing He","doi":"10.1021/acs.jafc.4c04883","DOIUrl":null,"url":null,"abstract":"Jasmonic acid (JA) is an endogenous phytohormone that regulates plant physiological metabolism and stress response processes, either independently or through hormone crosstalk. Our phytohormone assay and transcriptome–metabolome analysis revealed the key genes and metabolites involved in the JA pathway in response to 0–250 μM cadmium (Cd) in potato seedlings. Transcriptome gene set enrichment and gene ontology analysis indicated that JA-related genes were significantly enriched. Specifically, members from the <i>StOPR</i> and <i>StJAZ</i> gene families showed pronounced responses to Cd stress and methyl jasmonate treatment. As a negative regulatory transcription factor of the JA signaling pathway, <i>StJAZ14</i> exhibited a decreasing trend under Cd stress. Yeast two-hybrid assay identified an interaction between StJAZ14 and StBZR1, which is located on the brassinolide pathway. In addition to unveiling the critical role of the JA pathway in regulating potato response to Cd stress, the functional mechanism was preliminarily explored.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress\",\"authors\":\"Mingfang Yang, Sanwei Yang, Weidong Wang, Xiaoliao Wei, Fei Lou, GuanDi He, Tengbing He\",\"doi\":\"10.1021/acs.jafc.4c04883\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Jasmonic acid (JA) is an endogenous phytohormone that regulates plant physiological metabolism and stress response processes, either independently or through hormone crosstalk. Our phytohormone assay and transcriptome–metabolome analysis revealed the key genes and metabolites involved in the JA pathway in response to 0–250 μM cadmium (Cd) in potato seedlings. Transcriptome gene set enrichment and gene ontology analysis indicated that JA-related genes were significantly enriched. Specifically, members from the <i>StOPR</i> and <i>StJAZ</i> gene families showed pronounced responses to Cd stress and methyl jasmonate treatment. As a negative regulatory transcription factor of the JA signaling pathway, <i>StJAZ14</i> exhibited a decreasing trend under Cd stress. Yeast two-hybrid assay identified an interaction between StJAZ14 and StBZR1, which is located on the brassinolide pathway. In addition to unveiling the critical role of the JA pathway in regulating potato response to Cd stress, the functional mechanism was preliminarily explored.\",\"PeriodicalId\":41,\"journal\":{\"name\":\"Journal of Agricultural and Food Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2024-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Agricultural and Food Chemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.jafc.4c04883\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1021/acs.jafc.4c04883","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
茉莉酸(JA)是一种内源植物激素,可独立或通过激素串扰调节植物生理代谢和胁迫响应过程。我们的植物激素测定和转录组-代谢组分析揭示了马铃薯幼苗在 0-250 μM 镉(Cd)胁迫下参与 JA 通路的关键基因和代谢产物。转录组基因组富集和基因本体分析表明,JA相关基因显著富集。具体来说,StOPR 和 StJAZ 基因家族的成员对镉胁迫和茉莉酸甲酯处理有明显的反应。作为 JA 信号通路的负调控转录因子,StJAZ14 在镉胁迫下呈下降趋势。酵母双杂交试验发现了 StJAZ14 与位于黄铜素内酯通路上的 StBZR1 之间的相互作用。除了揭示JA通路在调控马铃薯对镉胁迫响应中的关键作用外,还初步探讨了其功能机制。
Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress
Jasmonic acid (JA) is an endogenous phytohormone that regulates plant physiological metabolism and stress response processes, either independently or through hormone crosstalk. Our phytohormone assay and transcriptome–metabolome analysis revealed the key genes and metabolites involved in the JA pathway in response to 0–250 μM cadmium (Cd) in potato seedlings. Transcriptome gene set enrichment and gene ontology analysis indicated that JA-related genes were significantly enriched. Specifically, members from the StOPR and StJAZ gene families showed pronounced responses to Cd stress and methyl jasmonate treatment. As a negative regulatory transcription factor of the JA signaling pathway, StJAZ14 exhibited a decreasing trend under Cd stress. Yeast two-hybrid assay identified an interaction between StJAZ14 and StBZR1, which is located on the brassinolide pathway. In addition to unveiling the critical role of the JA pathway in regulating potato response to Cd stress, the functional mechanism was preliminarily explored.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.