{"title":"用于核成像和外周血白细胞分拣的新一代红色超亮荧光染料","authors":"Zipeng Li, Zheng Liu, Ding Yu, Qichao Yao, Wanying Ma, Changyu Zhang, Jiangli Fan, Xiaojun Peng","doi":"10.1039/d4sc04848b","DOIUrl":null,"url":null,"abstract":"Nucleus is a membrane-bound organelle in eukaryotic cells and plays a crucial role in cellular processes. Visualizing nuclear morphology is essential for investigating nuclear functions and understanding the relationship between nuclear morphological alterations and multiple diseases. Fluorescent dyes have been developed to visualize nuclear morphology, but the selection of red nuclear-labeling fluorescent dyes remains limited (high price, unknown structure, or high toxicity). Herein, we have developed a red ultra-bright nuclear-targeted dye, <strong>BPC1</strong>, through the engineering of unsymmetrical cyanine dyes derived from D-π-A systems. <strong>BPC1</strong> exhibits ultrahigh fluorescence brightness and exceptional cell permeability, and selectively stains nuclear DNA rather than mitochondrial DNA, enabling the visualization of nucleus in diverse cells by extremely low doses (100 nM) and laser power (0.8 μW). Furthermore, <strong>BPC1</strong> is utilized for nuclear staining in blood cells, aiding in the distinct visualization of white blood cell nucleus and facilitating the identification and enumeration of various leukocyte types. Our study implies considerable commercial potential for <strong>BPC1</strong> and underscores its capacity to serve as a powerful tool in life sciences and cell biology research.","PeriodicalId":9909,"journal":{"name":"Chemical Science","volume":null,"pages":null},"PeriodicalIF":7.6000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Next-generation Red Ultra-bright Fluorescent Dyes for Nuclear Imaging and Peripheral Blood Leukocytes Sorting\",\"authors\":\"Zipeng Li, Zheng Liu, Ding Yu, Qichao Yao, Wanying Ma, Changyu Zhang, Jiangli Fan, Xiaojun Peng\",\"doi\":\"10.1039/d4sc04848b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Nucleus is a membrane-bound organelle in eukaryotic cells and plays a crucial role in cellular processes. Visualizing nuclear morphology is essential for investigating nuclear functions and understanding the relationship between nuclear morphological alterations and multiple diseases. Fluorescent dyes have been developed to visualize nuclear morphology, but the selection of red nuclear-labeling fluorescent dyes remains limited (high price, unknown structure, or high toxicity). Herein, we have developed a red ultra-bright nuclear-targeted dye, <strong>BPC1</strong>, through the engineering of unsymmetrical cyanine dyes derived from D-π-A systems. <strong>BPC1</strong> exhibits ultrahigh fluorescence brightness and exceptional cell permeability, and selectively stains nuclear DNA rather than mitochondrial DNA, enabling the visualization of nucleus in diverse cells by extremely low doses (100 nM) and laser power (0.8 μW). Furthermore, <strong>BPC1</strong> is utilized for nuclear staining in blood cells, aiding in the distinct visualization of white blood cell nucleus and facilitating the identification and enumeration of various leukocyte types. Our study implies considerable commercial potential for <strong>BPC1</strong> and underscores its capacity to serve as a powerful tool in life sciences and cell biology research.\",\"PeriodicalId\":9909,\"journal\":{\"name\":\"Chemical Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2024-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Science\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4sc04848b\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4sc04848b","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Next-generation Red Ultra-bright Fluorescent Dyes for Nuclear Imaging and Peripheral Blood Leukocytes Sorting
Nucleus is a membrane-bound organelle in eukaryotic cells and plays a crucial role in cellular processes. Visualizing nuclear morphology is essential for investigating nuclear functions and understanding the relationship between nuclear morphological alterations and multiple diseases. Fluorescent dyes have been developed to visualize nuclear morphology, but the selection of red nuclear-labeling fluorescent dyes remains limited (high price, unknown structure, or high toxicity). Herein, we have developed a red ultra-bright nuclear-targeted dye, BPC1, through the engineering of unsymmetrical cyanine dyes derived from D-π-A systems. BPC1 exhibits ultrahigh fluorescence brightness and exceptional cell permeability, and selectively stains nuclear DNA rather than mitochondrial DNA, enabling the visualization of nucleus in diverse cells by extremely low doses (100 nM) and laser power (0.8 μW). Furthermore, BPC1 is utilized for nuclear staining in blood cells, aiding in the distinct visualization of white blood cell nucleus and facilitating the identification and enumeration of various leukocyte types. Our study implies considerable commercial potential for BPC1 and underscores its capacity to serve as a powerful tool in life sciences and cell biology research.
期刊介绍:
Chemical Science is a journal that encompasses various disciplines within the chemical sciences. Its scope includes publishing ground-breaking research with significant implications for its respective field, as well as appealing to a wider audience in related areas. To be considered for publication, articles must showcase innovative and original advances in their field of study and be presented in a manner that is understandable to scientists from diverse backgrounds. However, the journal generally does not publish highly specialized research.