Nurdan Yazici , Seçkin Engin , Elif Nur Barut , Fadıl Kaan Kuran , Gozde Hasbal-Celikok , Tugba Yilmaz-Ozden , Mahmut Miski
{"title":"东方阿魏根的降血糖功效评估:从体外到体内的评估","authors":"Nurdan Yazici , Seçkin Engin , Elif Nur Barut , Fadıl Kaan Kuran , Gozde Hasbal-Celikok , Tugba Yilmaz-Ozden , Mahmut Miski","doi":"10.1016/j.fitote.2024.106225","DOIUrl":null,"url":null,"abstract":"<div><div>Decoctions of <em>Ferula orientalis</em> L. (Apiaceae), have been traditionally used to lower blood glucose levels (BGLs). After <em>in vitro</em> enzyme inhibition tests on the dichloromethane extracts of the roots (FOD) and the methanol extract of the roots (FOM), isolation studies were carried out on the FOD extract. The anti-hyperglycemic effects of the FOD extract and the pure compounds were studied in mice using the Oral Glucose Tolerance Test (OGTT) and streptozotocin (STZ)-induced diabetes mellitus (DM) models. Molecular docking studies were performed on potent compounds in the binding pockets of enzymes α-glucosidase and α-amylase. The isolations of 11 compounds were isolated from the FOD extract, which comprised teferidine (<strong>1</strong>), ferutinin (FT) (<strong>2</strong>), teferin (<strong>3</strong>), epoxy-jaeschkeanadiol-<em>p</em>-hydroxybenzoate (<strong>4</strong>), epoxy-jaeschkeanadiol-6-vanillate (<strong>5</strong>), tovarol-8-angelate (<strong>6</strong>), leucoferin (<strong>7</strong>), tovarol-8-<em>p</em>-hydroxybenzoate (<strong>8</strong>), tovarol-8-vanillate (<strong>9</strong>), 6-β-<em>p</em>-hydroxybenzoyloxy-germacra-1(10),4-diene (<strong>10</strong>), and chimgin (<strong>11</strong>). Compounds <strong>2</strong> and <strong>8</strong>–<strong>11</strong> exhibited a higher inhibitory activity on α-glucosidase. In the OGTT, pretreatment with the FOD extract or compound <strong>2</strong> did not alter the BGLs after administration of the glucose solution compared to the control. In the STZ-induced diabetic mice model, no significant difference in the BGLs was observed with the FOD extract (200 mg/kg) or compound <strong>2</strong> (100 mg/kg)-treated diabetic mice compared to the diabetic control mice. The experimental studies all showed that the <em>F. orientalis</em> extract had significant effects on the enzyme systems involved in DM, and it would be appropriate to plan further studies on possible problems of bioavailability of the compound FT and the FOD extract, inadequate dose, and duration of administration.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"179 ","pages":"Article 106225"},"PeriodicalIF":2.5000,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the Antihyperglycemic efficacy of the roots of Ferula orientalis L.: An in vitro to in vivo assessment\",\"authors\":\"Nurdan Yazici , Seçkin Engin , Elif Nur Barut , Fadıl Kaan Kuran , Gozde Hasbal-Celikok , Tugba Yilmaz-Ozden , Mahmut Miski\",\"doi\":\"10.1016/j.fitote.2024.106225\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Decoctions of <em>Ferula orientalis</em> L. (Apiaceae), have been traditionally used to lower blood glucose levels (BGLs). After <em>in vitro</em> enzyme inhibition tests on the dichloromethane extracts of the roots (FOD) and the methanol extract of the roots (FOM), isolation studies were carried out on the FOD extract. The anti-hyperglycemic effects of the FOD extract and the pure compounds were studied in mice using the Oral Glucose Tolerance Test (OGTT) and streptozotocin (STZ)-induced diabetes mellitus (DM) models. Molecular docking studies were performed on potent compounds in the binding pockets of enzymes α-glucosidase and α-amylase. The isolations of 11 compounds were isolated from the FOD extract, which comprised teferidine (<strong>1</strong>), ferutinin (FT) (<strong>2</strong>), teferin (<strong>3</strong>), epoxy-jaeschkeanadiol-<em>p</em>-hydroxybenzoate (<strong>4</strong>), epoxy-jaeschkeanadiol-6-vanillate (<strong>5</strong>), tovarol-8-angelate (<strong>6</strong>), leucoferin (<strong>7</strong>), tovarol-8-<em>p</em>-hydroxybenzoate (<strong>8</strong>), tovarol-8-vanillate (<strong>9</strong>), 6-β-<em>p</em>-hydroxybenzoyloxy-germacra-1(10),4-diene (<strong>10</strong>), and chimgin (<strong>11</strong>). Compounds <strong>2</strong> and <strong>8</strong>–<strong>11</strong> exhibited a higher inhibitory activity on α-glucosidase. In the OGTT, pretreatment with the FOD extract or compound <strong>2</strong> did not alter the BGLs after administration of the glucose solution compared to the control. In the STZ-induced diabetic mice model, no significant difference in the BGLs was observed with the FOD extract (200 mg/kg) or compound <strong>2</strong> (100 mg/kg)-treated diabetic mice compared to the diabetic control mice. The experimental studies all showed that the <em>F. orientalis</em> extract had significant effects on the enzyme systems involved in DM, and it would be appropriate to plan further studies on possible problems of bioavailability of the compound FT and the FOD extract, inadequate dose, and duration of administration.</div></div>\",\"PeriodicalId\":12147,\"journal\":{\"name\":\"Fitoterapia\",\"volume\":\"179 \",\"pages\":\"Article 106225\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fitoterapia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0367326X24004088\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0367326X24004088","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
摘要
东方阿魏(Apiaceae)的煎剂传统上用于降低血糖水平(BGLs)。在对根部二氯甲烷提取物(FOD)和根部甲醇提取物(FOM)进行体外酶抑制试验后,对 FOD 提取物进行了分离研究。利用口服葡萄糖耐量试验(OGTT)和链脲佐菌素(STZ)诱导的糖尿病(DM)模型,研究了 FOD 提取物和纯化合物对小鼠的抗高血糖作用。对α-葡萄糖苷酶和α-淀粉酶结合口袋中的强效化合物进行了分子对接研究。从 FOD 提取物中分离出了 11 种化合物,包括铁线蕨苷(1)、铁线蕨素(FT)(2)、铁线蕨素(3)、环氧-马齿苋二醇-对羟基苯甲酸酯(4)、环氧-马齿苋二醇-对羟基苯甲酸酯(5)、环氧-马齿苋二醇-对羟基苯甲酸酯(6)和环氧-马齿苋二醇-对羟基苯甲酸酯(7)、环氧-香豆素-6-香兰酸酯(5)、香豆素-8-angelate(6)、leucoferin(7)、香豆素-8-对羟基苯甲酸酯(8)、香豆素-8-香兰酸酯(9)、6-β-对羟基苯甲酰氧基-germacra-1(10),4-二烯(10)和 chimgin(11)。化合物 2 和 8-11 对α-葡萄糖苷酶具有较高的抑制活性。在 OGTT 试验中,与对照组相比,使用 FOD 提取物或化合物 2 进行预处理不会改变给予葡萄糖溶液后的 BGLs。在 STZ 诱导的糖尿病小鼠模型中,经 FOD 提取物(200 毫克/千克)或化合物 2(100 毫克/千克)处理的糖尿病小鼠与糖尿病对照组小鼠相比,BGLs 没有明显差异。这些实验研究都表明,东方鱼腥草提取物对参与 DM 的酶系统有明显的影响,因此应该计划进一步研究复方 FT 和 FOD 提取物的生物利用度、剂量不足和给药持续时间等可能存在的问题。
Evaluation of the Antihyperglycemic efficacy of the roots of Ferula orientalis L.: An in vitro to in vivo assessment
Decoctions of Ferula orientalis L. (Apiaceae), have been traditionally used to lower blood glucose levels (BGLs). After in vitro enzyme inhibition tests on the dichloromethane extracts of the roots (FOD) and the methanol extract of the roots (FOM), isolation studies were carried out on the FOD extract. The anti-hyperglycemic effects of the FOD extract and the pure compounds were studied in mice using the Oral Glucose Tolerance Test (OGTT) and streptozotocin (STZ)-induced diabetes mellitus (DM) models. Molecular docking studies were performed on potent compounds in the binding pockets of enzymes α-glucosidase and α-amylase. The isolations of 11 compounds were isolated from the FOD extract, which comprised teferidine (1), ferutinin (FT) (2), teferin (3), epoxy-jaeschkeanadiol-p-hydroxybenzoate (4), epoxy-jaeschkeanadiol-6-vanillate (5), tovarol-8-angelate (6), leucoferin (7), tovarol-8-p-hydroxybenzoate (8), tovarol-8-vanillate (9), 6-β-p-hydroxybenzoyloxy-germacra-1(10),4-diene (10), and chimgin (11). Compounds 2 and 8–11 exhibited a higher inhibitory activity on α-glucosidase. In the OGTT, pretreatment with the FOD extract or compound 2 did not alter the BGLs after administration of the glucose solution compared to the control. In the STZ-induced diabetic mice model, no significant difference in the BGLs was observed with the FOD extract (200 mg/kg) or compound 2 (100 mg/kg)-treated diabetic mice compared to the diabetic control mice. The experimental studies all showed that the F. orientalis extract had significant effects on the enzyme systems involved in DM, and it would be appropriate to plan further studies on possible problems of bioavailability of the compound FT and the FOD extract, inadequate dose, and duration of administration.
期刊介绍:
Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas:
1. Characterization of active ingredients of medicinal plants
2. Development of standardization method for bioactive plant extracts and natural products
3. Identification of bioactivity in plant extracts
4. Identification of targets and mechanism of activity of plant extracts
5. Production and genomic characterization of medicinal plants biomass
6. Chemistry and biochemistry of bioactive natural products of plant origin
7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.