miR-98 和 miR-629 可用作复发缓解型多发性硬化症患者的潜在生物标记物

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-09-19 DOI:10.1016/j.genrep.2024.102041

Burçin Erkal Çam , Tuğba Elgün , Tuğba Kul Köprülü , Arzu Çoban , Şenay Vural Korkut

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引用次数: 0

摘要

导言多发性硬化症（MS）是一种影响中枢神经系统（CNS）的自身免疫性疾病，以慢性炎症、脱髓鞘和神经变性为特征。近年来，在非编码 RNA 中，miRNA 已成为不同生物过程的关键调控因子，并被认为在多发性硬化症的发病机制中发挥着重要作用。通过硅学方法，miR-629-5p 和 miR-98-5p 被确定为多发性硬化症病理的重要因素。本研究旨在检测复发性多发性硬化症患者血液样本中 miR-629-5p 和 miR-98-5p 的表达水平。研究方法采集 RRMS 组和对照组患者的总血样，采用 qPCR 分析评估目标 miRNAs 的表达。利用 mirDIP 数据库确定靶基因。利用 Cytoscape 确定枢纽基因，利用 STRING 确定目标通路。结果显示表达分析表明，在 RRMS 患者中，miR-629-5p 和 miR-98-5p 表达明显上调（FC ≥ 1.5，p < 0.05）。PI3K-Akt、Rap1、MAPK和FoxO信号通路被认为是靶点。讨论这些 miRNAs 的发现表明，它们可能通过干预免疫系统和神经系统的通路对多发性硬化症患者产生显著影响。

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miR-98 and miR-629 can be used as a potential biomarker on relapsing-remitting multiple sclerosis patients

Introduction

Multiple Sclerosis (MS) is an autoimmune disease that affects the central nervous system (CNS), characterized by chronic inflammation, demyelination, and neurodegeneration. In recent years, among non-coding RNAs miRNA have emerged as key regulators of different biological processes and it has been suggested that they play an important role in the mechanisms underlying MS pathogenesis. Through in silico methods, miR-629-5p and miR-98-5p were identified as significant factors in MS pathology. The aim of this study was to examine the levels of expression of miR-629-5p and miR-98-5p in blood samples obtained from patients with relapsing–remitting MS. Methods: Total blood were recruited from RRMS and control group and qPCR analysis was used for evaluating of target miRNAs expression. The mirDIP database was utilized to identify the target genes. Hub genes were identified with the Cytoscape and target pathways were identified using the STRING. Results: Expression analysis revealed a significant upregulation of miR-629-5p and miR-98-5p (FC ≥ 1.5 and p < 0.05) in patients with RRMS. PI3K-Akt, Rap1, MAPK and FoxO signaling pathways were found as target. Discussion: The discovery of these miRNAs suggests that they may have a notable impact on MS patients by intervening on pathways involved in both the immune and nervous systems.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.