加纳非洲茄子(Solanum aethiopicum)冠腐病和枯萎病相关镰刀菌和新孢子菌菌种的特征描述

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES
Benjamin Azu Okorley, Sabine Ravnskov, Francis C. Brentu, Samuel K. Offei
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引用次数: 0

摘要

非洲茄子(Solanum aethiopicum group Gilo)是加纳和热带非洲的一种重要蔬菜,具有相当高的经济价值。然而,真菌病害威胁着它的种植。2021 年和 2022 年生长季节在加纳五个地区的 35 个商业农场进行的调查显示,冠腐病和枯萎病的症状影响了 AEP。本研究采用形态学、分子和致病性检测方法,对导致 AEPs 发生这些病害的 36 个真菌分离物进行了鉴定和特征描述。通过对 Btub2、Tef-1α 和 Rpb2 序列进行形态学和分子分析,确定了与植物病害相关的两个镰刀菌种(F. elaeidis 和 F. fredkrugeri)和三个新孢子菌种(N. falciforme、N. suttoniana 和 N. solani)。F.elaeidis(14 个分离物)和 N. falciforme(14 个)是从有症状植物中分离出来的最常见物种。具体来说,枯萎植株中发现了 F. elaeidis,而 F. fredkrugeri 和三种 Neocosmospora 菌属则更多地与枯萎植株和冠腐病症状有关,而不是与仅有枯萎症状的植株有关。当接种到 AEP 的根部和茎部时,所有确定的物种都表现出致病性,这证实了实地观察结果。F.elaeidis在诱导枯萎病症状方面最具侵染性,而N. solani和N. suttoniana在诱导冠腐病症状方面尤其具有侵染性。这项研究首次证明,F. elaeidis、F. fredkrugeri、N. falciforme 和 N. suttoniana 是导致加纳 AEP 枯萎病和冠腐病的病原体。这些发现为制定有效的病害管理策略以减少这些真菌造成的损失提供了重要启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterisation of Fusarium and Neocosmospora Species Associated With Crown Rot and Wilt of African Eggplant (Solanum aethiopicum) in Ghana

Characterisation of Fusarium and Neocosmospora Species Associated With Crown Rot and Wilt of African Eggplant (Solanum aethiopicum) in Ghana

African eggplant (AEP) (Solanum aethiopicum group Gilo) is an important vegetable with considerable economic value in Ghana and tropical Africa. However, fungal diseases threaten its cultivation. Surveys conducted in 2021 and 2022 growing seasons across 35 commercial farms in five regions of Ghana revealed symptoms of crown rot and wilt affecting AEP. This study was undertaken to identify and characterise 36 fungal isolates causing these diseases in AEPs using morphological, molecular and pathogenicity assays. Morphological and molecular analyses of the Btub2, Tef-1α and Rpb2 sequences identified two Fusarium species (F. elaeidis and F. fredkrugeri) and three Neocosmospora species (N. falciforme, N. suttoniana and N. solani) associated with the plant diseases. F. elaeidis (14 isolates) and N. falciforme (14) were the most commonly isolated species from symptomatic plants. Specifically, F. elaeidis was found in wilting plants, while F. fredkrugeri and the three Neocosmospora spp. were more associated with wilting plants with crown rot symptoms than plants with only wilt symptoms. All identified species exhibited pathogenicity when inoculated onto AEP roots and stems, confirming field observations. F. elaeidis was the most aggressive in inducing wilt symptoms, while N. solani and N. suttoniana were particularly aggressive in inducing crown rot symptoms. This study is the first to document that F. elaeidis, F. fredkrugeri, N. falciforme and N. suttoniana are pathogens causing wilt and crown rot in AEP in Ghana. These findings provide essential insights for developing effective disease management strategies to reduce losses from these fungal species.

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来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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