开发用于快速检测人体尿液中 11-脱氢色腺素 B2 的免疫层析试纸条

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-09-01 DOI:10.1016/j.cjac.2024.100436

Guangxing TAN , Xiaoqian JIANG , Chuanlai XU , Zhuyuan FANG

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引用次数: 0

摘要

尿液中的 11-脱氢血色素 B2（11-DH-TXB2）是评估心血管疾病患者接受阿司匹林治疗效果的诊断指标。因此，开发尿液中 11-DH-TXB2 的快速检测方法具有重要的临床意义。本研究制备了一种针对 11-DH-TXB2 的超灵敏单克隆抗体（mAb）。该 mAb 属于带有 kappa 轻链的 IgG1 亚型。在间接竞争性酶联免疫吸附试验中，它的半最大抑制浓度为 3.63 纳克/毫升，对 11-DH-TXB2 的亲和力高达 5.60 × 109 L/mol。随后，我们开发了一种免疫层析试纸条，用于对人体尿液中的 11-DH-TXB2 进行床旁检测（POCT）。该试剂条的临界值和检测限分别为 500 纳克/毫升和 31.2 纳克/毫升。15 分钟内即可得出结果。总之，该试剂条是一种监测人体尿液中 11-DH-TXB2 的便携式 POCT 工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Development of an immunochromatographic strip for the rapid detection of 11-dehydro-thromboxane B2 in human urine

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Development of an immunochromatographic strip for the rapid detection of 11-dehydro-thromboxane B2 in human urine

Urinary 11-dehydro-thromboxane B2 (11-DH-TXB2) is a diagnostic marker for evaluating the effect of aspirin treatment in patients with cardiovascular disease. Therefore, it is of great clinical significance to develop a rapid detection method for 11-DH-TXB2 in urine. In this study, we prepared an ultrasensitive monoclonal antibody (mAb) against 11-DH-TXB2. The mAb belongs to the IgG1 subtype with kappa light chains. In an indirect competitive ELISA, it had a half-maximal inhibitory concentration of 3.63 ng/mL and a high affinity of 5.60 × 10⁹ L/mol for 11-DH-TXB2. We then developed an immunochromatographic strip for point-of-care testing (POCT) of 11-DH-TXB2 in human urine. The strip provides a cut-off value and limit of detection of 500 ng/mL and 31.2 ng/mL, respectively. The result is obtained within 15 min. In summary, the proposed strip is a portable POCT tool for monitoring 11-DH-TXB2 in human urine.

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.