作为骨骼肌衰老标志的管状聚集体

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-09-25 DOI:10.1007/7651_2024_567

Felipe Tadeu Galante Rocha de Vasconcelos, Brandow Willy Souza, Lucas Santos Souza, Mariz Vainzof

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引用次数: 0

摘要

管状聚集体（TA）是一种骨骼肌结构，主要是随着年龄的增长，肌浆网蛋白质逐渐积累而成。肌肉再生在TA形成过程中起一定作用。TA定量有助于评估肌肉衰老和遗传性肌肉退化。TA随着时间的推移而形成，出现在正常小鼠肌肉的衰老过程中。在受伤的情况下，TA 的减少可能是由于肌肉的变性-再生过程，即受损肌纤维的损失和不出现蛋白质聚集的新纤维的形成。这些新的再生纤维并不能提高老化肌肉的功能能力。在此，我们提出了一种标记和识别肌肉纤维中管状聚集体的方法，并对其量化进行了标准化。

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Tubular Aggregates as a Marker of Aging in Skeletal Muscle.

Tubular aggregates (TA) are skeletal muscle structures that arise from the progressive accumulation of sarcoplasmic reticulum proteins, mainly with aging. Muscle regeneration plays a role in TA formation. TA quantification may aid in the evaluation of muscle aging and genetic muscle degeneration. TA form over time, appears in aging in normal murine muscles. TA reduction in injured conditions may be due to the degeneration-regeneration process in muscles, with loss of damaged muscle fibers and formation of new fibers that do not present protein aggregation. These new regenerated fibers do not improve the function capacity of the aged muscle. Here, we present a methodology for labeling and identifying tubular aggregates in muscle fibers and also the standardization of its quantification.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.