{"title":"胃泌素 rs26311 和 rs27647 多态性及 mRNA 表达与子痫前期易感性和严重程度的关系--一项病例对照研究。","authors":"Farzaneh Montazerifar, Saeedeh Salimi, Rasul Taghvaeefar, Mansour Karajibani, Marzieh Ghasemi, Mehrnaz Mehrabani, Mahnaz Rezaei","doi":"10.1590/1806-9282.20231638","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Ghrelin is an adipokine the placenta generates to control the maternal metabolic adaptation to pregnancy. It causes different pregnancy complications like preeclampsia (PE). Therefore, the aim of this study was to assess the association between ghrelin mRNA expression and rs26311 and rs27647 polymorphisms and PE development.</p><p><strong>Methods: </strong>In total, 156 PE women (including 97 patients with mild PE and 59 patients with severe PE) and 152 healthy controls were recruited in this case-control study during 2019-2020. All participants with other diseases have been excluded from both groups. The ghrelin expression was analyzed with real-time PCR, and ghrelin variants were examined using the RFLP-PCR method.</p><p><strong>Results: </strong>The maternal and placental ghrelin rs27647 and rs26311 variants were unrelated to PE susceptibility. Haplotype analyses showed no significant difference between the four haplotypes and PE. No relationship was revealed between rs27647 polymorphism and severe PE. However, the results indicated a relationship between rs27647 and severe PE compared to mild PE and controls. Therefore, the rs27647 variant was associated with severe PE compared to mild PE in codominant, recessive, and log-additive models and controls in codominant, dominant, recessive, and log-additive models. The placental ghrelin mRNA expression declined in PE women compared to controls (0.67-fold), but the difference was insignificant (p=0.263). No significant difference was found between various genotypes of rs27647 and rs26311 polymorphisms concerning ghrelin mRNA expression.</p><p><strong>Conclusion: </strong>The maternal and placental ghrelin polymorphisms, rs27647 and rs26311, showed no effect on PE. However, the rs27647 variant was associated with severe PE.</p>","PeriodicalId":94194,"journal":{"name":"Revista da Associacao Medica Brasileira (1992)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404987/pdf/","citationCount":"0","resultStr":"{\"title\":\"The association of ghrelin rs26311 and rs27647 polymorphisms and mRNA expression with preeclampsia susceptibility and severity-A case-control study.\",\"authors\":\"Farzaneh Montazerifar, Saeedeh Salimi, Rasul Taghvaeefar, Mansour Karajibani, Marzieh Ghasemi, Mehrnaz Mehrabani, Mahnaz Rezaei\",\"doi\":\"10.1590/1806-9282.20231638\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Ghrelin is an adipokine the placenta generates to control the maternal metabolic adaptation to pregnancy. It causes different pregnancy complications like preeclampsia (PE). Therefore, the aim of this study was to assess the association between ghrelin mRNA expression and rs26311 and rs27647 polymorphisms and PE development.</p><p><strong>Methods: </strong>In total, 156 PE women (including 97 patients with mild PE and 59 patients with severe PE) and 152 healthy controls were recruited in this case-control study during 2019-2020. All participants with other diseases have been excluded from both groups. The ghrelin expression was analyzed with real-time PCR, and ghrelin variants were examined using the RFLP-PCR method.</p><p><strong>Results: </strong>The maternal and placental ghrelin rs27647 and rs26311 variants were unrelated to PE susceptibility. Haplotype analyses showed no significant difference between the four haplotypes and PE. No relationship was revealed between rs27647 polymorphism and severe PE. However, the results indicated a relationship between rs27647 and severe PE compared to mild PE and controls. Therefore, the rs27647 variant was associated with severe PE compared to mild PE in codominant, recessive, and log-additive models and controls in codominant, dominant, recessive, and log-additive models. The placental ghrelin mRNA expression declined in PE women compared to controls (0.67-fold), but the difference was insignificant (p=0.263). No significant difference was found between various genotypes of rs27647 and rs26311 polymorphisms concerning ghrelin mRNA expression.</p><p><strong>Conclusion: </strong>The maternal and placental ghrelin polymorphisms, rs27647 and rs26311, showed no effect on PE. However, the rs27647 variant was associated with severe PE.</p>\",\"PeriodicalId\":94194,\"journal\":{\"name\":\"Revista da Associacao Medica Brasileira (1992)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404987/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista da Associacao Medica Brasileira (1992)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/1806-9282.20231638\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista da Associacao Medica Brasileira (1992)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1806-9282.20231638","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的胃泌素是胎盘产生的一种脂肪因子,用于控制母体对妊娠的代谢适应。它可导致不同的妊娠并发症,如子痫前期(PE)。因此,本研究旨在评估 ghrelin mRNA 表达、rs26311 和 rs27647 多态性与 PE 发生之间的关联:本病例对照研究在2019-2020年间共招募了156名PE女性(包括97名轻度PE患者和59名重度PE患者)和152名健康对照者。两组中均排除了所有患有其他疾病的参与者。采用实时荧光定量PCR分析胃泌素的表达,并采用RFLP-PCR方法检测胃泌素变异:结果:母体和胎盘胃泌素 rs27647 和 rs26311 变体与 PE 易感性无关。单倍型分析表明,四种单倍型与 PE 没有显著差异。rs27647 多态性与重度 PE 之间没有关系。然而,结果表明,与轻度 PE 和对照组相比,rs27647 与重度 PE 有一定关系。因此,在显性、隐性和对数相加模型中,与轻度 PE 相比,rs27647 变异与重度 PE 相关;在显性、显性、隐性和对数相加模型中,与对照组相比,rs27647 变异与重度 PE 相关。与对照组相比,PE 妇女的胎盘胃泌素 mRNA 表达下降(0.67 倍),但差异不显著(P=0.263)。rs27647和rs26311多态性的不同基因型在胃泌素mRNA表达方面无明显差异:结论:母体和胎盘胃泌素多态性 rs27647 和 rs26311 对 PE 没有影响。然而,rs27647变异与重度PE有关。
The association of ghrelin rs26311 and rs27647 polymorphisms and mRNA expression with preeclampsia susceptibility and severity-A case-control study.
Objective: Ghrelin is an adipokine the placenta generates to control the maternal metabolic adaptation to pregnancy. It causes different pregnancy complications like preeclampsia (PE). Therefore, the aim of this study was to assess the association between ghrelin mRNA expression and rs26311 and rs27647 polymorphisms and PE development.
Methods: In total, 156 PE women (including 97 patients with mild PE and 59 patients with severe PE) and 152 healthy controls were recruited in this case-control study during 2019-2020. All participants with other diseases have been excluded from both groups. The ghrelin expression was analyzed with real-time PCR, and ghrelin variants were examined using the RFLP-PCR method.
Results: The maternal and placental ghrelin rs27647 and rs26311 variants were unrelated to PE susceptibility. Haplotype analyses showed no significant difference between the four haplotypes and PE. No relationship was revealed between rs27647 polymorphism and severe PE. However, the results indicated a relationship between rs27647 and severe PE compared to mild PE and controls. Therefore, the rs27647 variant was associated with severe PE compared to mild PE in codominant, recessive, and log-additive models and controls in codominant, dominant, recessive, and log-additive models. The placental ghrelin mRNA expression declined in PE women compared to controls (0.67-fold), but the difference was insignificant (p=0.263). No significant difference was found between various genotypes of rs27647 and rs26311 polymorphisms concerning ghrelin mRNA expression.
Conclusion: The maternal and placental ghrelin polymorphisms, rs27647 and rs26311, showed no effect on PE. However, the rs27647 variant was associated with severe PE.