姜黄素纳米乳剂对环磷酰胺诱导的成年雄性小鼠睾丸毒性的治疗作用

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Pourya Raee, Shahin Aghamiri, Mahsa Ghaffari Novin, Azar Afshar, Fakhroddin Aghajanpour, Farid Abdi, Marefat Ghaffari Novin
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引用次数: 0

摘要

目的:包括环磷酰胺(CP)和丁硫丹在内的几种化疗药物已被证明会干扰精子发生。因此,本研究的主要目的是评估姜黄素纳米乳剂(CUR-NE)对 CP 诱导的睾丸毒性小鼠精子发生的潜在治疗作用:将28只成年雄性小鼠平均分为四组:对照组、CUR-NE组(30毫克/千克,每天一次,连续5周)、CP组(200毫克/千克,单剂量)和CP+CUR-NE组。每组都对精子参数、DNA碎片指数、染色质成熟度、活性氧(ROS)水平和睾丸组织学参数进行了评估。此外,还评估了所有组的卵泡刺激素(FSH)、黄体生成素和睾酮的血清水平:结果:在CP诱导的小鼠中,CUR-NE能显著改善精子参数,包括精子总数、活力、形态和DNA完整性。服用 CUR-NE 还能显著提高 CP 治疗小鼠的睾酮和 FSH 血清水平,以及睾丸重量和体积。此外,CUR-NE 还能显著增加这些动物睾丸组织中精原细胞、初级精母细胞、圆形精子细胞和雷迪格细胞的数量。CP诱导的小鼠服用 CUR-NE 后,睾丸组织中的 ROS 水平明显下降:结论:CUR-NE 似乎能通过降低 ROS 水平、改善睾丸立体学参数和增强生殖激素谱来促进 CP 诱导的睾丸毒性小鼠的精子发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Therapeutic effects of curcumin nanoemulsion on cyclophosphamide-induced testicular toxicity in adult male mice.

Objective: Several chemotherapeutic agents, including cyclophosphamide (CP) and busulfan, have been shown to interfere with spermatogenesis. Accordingly, the main objective of this study was to evaluate the potential therapeutic effects of curcumin nanoemulsion (CUR-NE) on spermatogenesis in mice with CP-induced testicular toxicity.

Methods: A total of 28 adult male mice were equally divided into four groups: control, CUR-NE (30 mg/kg, daily for 5 weeks), CP (200 mg/kg, single dose), and CP+CUR-NE. Each group was evaluated regarding sperm parameters, DNA fragmentation index, chromatin maturation, reactive oxygen species (ROS) levels, and histological parameters of the testes. Serum levels of follicle-stimulating hormone (FSH), luteinizing hormone, and testosterone were also assessed in all groups.

Results: In CP-induced mice, CUR-NE treatment significantly improved sperm parameters, including total sperm count, motility, morphology, and DNA integrity. CUR-NE administration was also associated with significantly higher serum levels of testosterone and FSH, as well as testis weight and volume, in the mice treated with CP. Furthermore, CUR-NE treatment significantly increased the number of spermatogonia, primary spermatocytes, round spermatids, and Leydig cells in the testicular tissue of these animals. A marked reduction in ROS levels in the testes tissue was observed following administration of CUR-NE to CP-induced mice.

Conclusion: CUR-NE appears to promote spermatogenesis in mice with CP-induced testicular toxicity by reducing ROS levels, improving testicular stereological parameters, and strengthening the reproductive hormone profile.

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