在新生大鼠体外制备中，内啡肽-2（Endo2）和P物质（SubP）联合应用可减轻SubP诱导的兴奋并改变频率可塑性。

IF 1.9 4区医学 Q3 PHYSIOLOGY

Respiratory Physiology & Neurobiology Pub Date : 2024-09-19 DOI:10.1016/j.resp.2024.104351

Stephen M. Johnson, Sarah M. Johnson, Jyoti J. Watters, Tracy L. Baker

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引用次数: 0

摘要

物质 P（SubP）和内吗啡肽-2（Endo2）共同定位在邻近吸气节律产生前博钦格复合体（preBotC）神经元的突触前囊泡中，但共同释放的 SubP 和 Endo2 对呼吸运动控制的影响尚不清楚。为了解决这个问题，我们在新生大鼠脑干脊髓制备物中以 10-100 nM 的剂量持续（15 分钟）或间歇（5 分钟施用，5 分钟冲洗，x3）沐浴施用 SubP 或 SubP 和 Endo2 的组合（SubP/Endo2）。在应用神经肽的过程中，SubP/Endo2 联合应用通常会减弱 SubP 引起的爆发频率增加和爆发振幅减小。关于频率可塑性（应用神经肽后 60 分钟爆发频率的持久增加），SubP/Endo2 在 20 和 100 nM 的浓度下持续联合应用会增加 SubP 诱导的频率可塑性。间歇施用 SubP/Endo2 有降低间歇单独施用 SubP 诱导的频率可塑性水平的趋势。SubP/Endo2联合应用揭示了神经激肽-1（NK1R）和μ-阿片（MOR）受体在呼吸节律生成延髓神经元上的潜在新功能。

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Endomorphin-2 (Endo2) and substance P (SubP) co-application attenuates SubP-induced excitation and alters frequency plasticity in neonatal rat in vitro preparations

Substance P (SubP) and endomorphin-2 (Endo2) are co-localized presynaptically in vesicles of neurons adjacent to inspiratory rhythm-generating pre-Botzinger Complex (preBotC) neurons but the effects of co-released SubP and Endo2 on respiratory motor control are not known. To address this question, SubP alone or a combination of SubP and Endo2 (SubP/Endo2) were bath-applied in a sustained (15-min) or intermittent (5-min application, 5-min washout, x3) pattern at 10–100 nM to neonatal rat brainstem-spinal cord preparations. During neuropeptide application, SubP/Endo2 co-applications generally attenuated SubP-induced increases in burst frequency and decreases in burst amplitude. With respect to frequency plasticity (long-lasting increase in burst frequency 60 min post-neuropeptide application), SubP-induced frequency plasticity was increased with sustained SubP/Endo2 co-applications at 20 and 100 nM. Intermittent SubP/Endo2 co-applications tended to decrease the level of frequency plasticity induced by intermittent SubP alone applications. SubP/Endo2 co-applications revealed potentially new functions for neurokinin-1 (NK1R) and mu-opioid (MOR) receptors on respiratory rhythm-generating medullary neurons.

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来源期刊

Respiratory Physiology & Neurobiology 医学-呼吸系统

CiteScore

4.80

自引率

8.70%

发文量

104

审稿时长

54 days

期刊介绍： Respiratory Physiology & Neurobiology (RESPNB) publishes original articles and invited reviews concerning physiology and pathophysiology of respiration in its broadest sense. Although a special focus is on topics in neurobiology, high quality papers in respiratory molecular and cellular biology are also welcome, as are high-quality papers in traditional areas, such as: -Mechanics of breathing- Gas exchange and acid-base balance- Respiration at rest and exercise- Respiration in unusual conditions, like high or low pressure or changes of temperature, low ambient oxygen- Embryonic and adult respiration- Comparative respiratory physiology. Papers on clinical aspects, original methods, as well as theoretical papers are also considered as long as they foster the understanding of respiratory physiology and pathophysiology.