通过多重免疫荧光染色对图像进行定量分析，从而对人类胎盘中的相关标记物进行细胞类型特异性空间表型分析。

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta Pub Date : 2024-09-18 DOI:10.1016/j.placenta.2024.09.012

Theresa Forndran, Silke Große, Gina Weber, Lara Hausdorf, Dagmar Samsel, Alexander Berndt, Nikolaus Gaßler, Tanja Groten

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引用次数: 0

摘要

与其他组织不同，胎盘由许多功能不同的细胞类型组成，这些细胞类型在胎盘内部和不同病例之间的分布明显不同。为了评估细胞表型和分子标记物表达的病理特异性变化，必须建立一种多重染色方法，将细胞类型的免疫组织学鉴定与相关蛋白的染色结合起来。我们成功建立了一套用于多重免疫荧光的 6 重抗体面板方案。在此，我们报告了染色方案以及我们开发的量化算法的建立情况。

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Protocol for the quantitative analysis of images retrieved by multiplex immunofluorescence staining to allow cell type-specific spatial phenotyping of markers of interest in the human placenta.

In contrast to other tissues, the placenta consists of numerous functionally different cell types, distributed in a markedly dissimilar manner within one placenta and between different cases. To evaluate pathology-specific changes in cell phenotype and expression of molecular markers it is important to establish a multi staining method combining immunohistological identification of the cell type with staining of proteins of interest. We successfully established a protocol for a 6-plex antibody panel for multiplex immunofluorescence. Here, we report the staining protocol and the establishment of the quantification algorithm we developed.

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来源期刊

Placenta 医学-发育生物学

CiteScore

6.30

自引率

10.50%

发文量

391

审稿时长

78 days

期刊介绍： Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.