{"title":"以 T98 脑胶质瘤细胞为研究对象,在体外生物测定指导下鉴定荆芥叶中的抗癌化合物。叶中的抗癌化合物。","authors":"Sunitee Hansdah, Shubhasmita Mohapatra, Kabita Khuntia, Anil Kumar Biswal, Priya Ranjan Debata","doi":"10.1080/14786419.2024.2404647","DOIUrl":null,"url":null,"abstract":"<p><p>Gliomas, the most devastating of brain tumours, pose immense therapeutic challenges due to the adverse effects of standard chemotherapeutic drugs. Seeking alternatives, natural products have emerged as promising sources for cancer treatment. <i>Vitex negundo</i>, a significant medicinal plant in traditional medicine, offers potential remedies for various ailments. In this study, fractionation <i>via</i> column chromatography we isolated fraction #25 from leaves of Vitex negundo. Employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, flow cytometry for cell cycle analysis, and HRLC-MS for compound identification, we investigated its effects on glioma cells. Results indicate that fraction #25 significantly reduces glioma cell viability and proliferation, inhibits cell migration in a dose-dependent manner, and arrests the cell cycle at G1 phase. Compound analysis reveals the presence of potent antiproliferative agents, including 7-hydroxy-3,4,5,6,8-pentamethoxyflavone, Virol-B, Momordin Ia, and Oryzalexin A. These findings underscore the potential of <i>Vitex negundo</i> as a source of anticancer compounds against glioma cells.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro, bioassay guided identification of anticancer compounds from <i>Vitex negundo</i> Linn. Leaf using T98 glioma cells.\",\"authors\":\"Sunitee Hansdah, Shubhasmita Mohapatra, Kabita Khuntia, Anil Kumar Biswal, Priya Ranjan Debata\",\"doi\":\"10.1080/14786419.2024.2404647\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Gliomas, the most devastating of brain tumours, pose immense therapeutic challenges due to the adverse effects of standard chemotherapeutic drugs. Seeking alternatives, natural products have emerged as promising sources for cancer treatment. <i>Vitex negundo</i>, a significant medicinal plant in traditional medicine, offers potential remedies for various ailments. In this study, fractionation <i>via</i> column chromatography we isolated fraction #25 from leaves of Vitex negundo. Employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, flow cytometry for cell cycle analysis, and HRLC-MS for compound identification, we investigated its effects on glioma cells. Results indicate that fraction #25 significantly reduces glioma cell viability and proliferation, inhibits cell migration in a dose-dependent manner, and arrests the cell cycle at G1 phase. Compound analysis reveals the presence of potent antiproliferative agents, including 7-hydroxy-3,4,5,6,8-pentamethoxyflavone, Virol-B, Momordin Ia, and Oryzalexin A. These findings underscore the potential of <i>Vitex negundo</i> as a source of anticancer compounds against glioma cells.</p>\",\"PeriodicalId\":18990,\"journal\":{\"name\":\"Natural Product Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/14786419.2024.2404647\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2024.2404647","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
In vitro, bioassay guided identification of anticancer compounds from Vitex negundo Linn. Leaf using T98 glioma cells.
Gliomas, the most devastating of brain tumours, pose immense therapeutic challenges due to the adverse effects of standard chemotherapeutic drugs. Seeking alternatives, natural products have emerged as promising sources for cancer treatment. Vitex negundo, a significant medicinal plant in traditional medicine, offers potential remedies for various ailments. In this study, fractionation via column chromatography we isolated fraction #25 from leaves of Vitex negundo. Employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, flow cytometry for cell cycle analysis, and HRLC-MS for compound identification, we investigated its effects on glioma cells. Results indicate that fraction #25 significantly reduces glioma cell viability and proliferation, inhibits cell migration in a dose-dependent manner, and arrests the cell cycle at G1 phase. Compound analysis reveals the presence of potent antiproliferative agents, including 7-hydroxy-3,4,5,6,8-pentamethoxyflavone, Virol-B, Momordin Ia, and Oryzalexin A. These findings underscore the potential of Vitex negundo as a source of anticancer compounds against glioma cells.
期刊介绍:
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.