评估伴有或不伴有 2 型糖尿病的牙周炎患者唾液中的白细胞介素-17 和发育内皮基因座-1。

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Yong Zhang, Xu Su, Yuzhi Li, Yu Cai, Ni Kang, Jinyu Duan, Fan Chen, Fei Xue, Xiaotao Chen
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Periodontal parameters (plaque index [PI], bleeding on probing [BOP], probing depth [PD], and clinical attachment level [CAL]) as well as diabetic parameters (fasting plasma glucose [FG] and glycated hemoglobin [HbA1c]) were documented and unstimulated saliva was collected. Salivary IL-1β, active-matrix metalloproteinase-8 (aMMP-8), tumor necrosis factor-α (TNF-α), IL-6, IL-8, IL-17, and Del-1 were determined through enzyme-linked immunosorbent assay (ELISA) and their relationships with periodontal and diabetic parameters were examined.</p><p><strong>Results: </strong>The periodontitis and periodontitis with diabetes groups showed significantly higher levels of IL-17 and lower levels of Del-1 compared with healthy group. The periodontitis with diabetes group exhibited higher levels of IL-17 and lower levels of Del-1 compared with the periodontitis group. After correlation analysis, there were significant correlations between salivary IL-17 and Del-1 and clinical parameters, IL-17 and Del-1 were correlated with PD (r = 0.36, -0.39, p < 0.01), CAL (r = 0.40, -0.42, p < 0.01) and BOP (r = 0.35, -0.37, p < 0.01), they were correlated with FG (r = 0.26, -0.25, p < 0.05) and HbA1c (r = 0.28, -0.40, p < 0.05). Positive relationships were observed between IL-17 and IL-1β and between IL-17 and aMMP-8 (r = 0.80, 0.77, p < 0.01), while Del-1 exhibited negative correlations with IL-1β and aMMP-8 (r = 0.59, 0.69 p < 0.01). Comparison between IL-17 and Del-1 confirmed an inverse relationship (r = -0.71, p < 0.01). 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引用次数: 0

摘要

背景:白细胞介素17(IL-17)和发育内皮位点-1(Del-1)被认为在牙周炎和T2DM中发挥作用。本研究旨在通过测量牙周炎患者和非 T2DM 患者的唾液水平,研究 IL-17 和 Del-1 的相关性:方法: 共有 80 人参加了横断面研究,并根据牙周和糖尿病检查结果分为健康组(H,n = 27)、牙周炎组(P,n = 29)和牙周炎合并糖尿病组(PDM,n = 24)。记录牙周参数(菌斑指数[PI]、探诊出血[BOP]、探诊深度[PD]和临床附着水平[CAL])和糖尿病参数(空腹血浆葡萄糖[FG]和糖化血红蛋白[HbA1c]),并收集未刺激唾液。通过酶联免疫吸附试验(ELISA)测定唾液中的IL-1β、活性基质金属蛋白酶-8(aMMP-8)、肿瘤坏死因子-α(TNF-α)、IL-6、IL-8、IL-17和Del-1,并研究它们与牙周和糖尿病参数的关系:结果:与健康组相比,牙周炎组和牙周炎伴糖尿病组的 IL-17 水平明显较高,而 Del-1 水平较低。与牙周炎组相比,牙周炎伴糖尿病组的 IL-17 水平更高,Del-1 水平更低。经过相关分析,唾液中的IL-17和Del-1与临床指标有显著相关性,IL-17和Del-1与PD相关(r = 0.36,-0.39,P 0.05):结论:与牙周炎组相比,牙周炎合并糖尿病组患者唾液中的IL-17和Del-1水平有显著变化,两者呈反比关系,且均与牙周参数(PD、CAL和BOP)和糖尿病参数(FG和HbA1c)相关。白细胞介素 17 [IL-17] 和发育内皮位点-1 [Del-1])调控中性粒细胞的产生、募集和清除,被认为与牙周炎和糖尿病的进展有关。在这项研究中,我们招募了健康受试者、牙周炎患者、牙周炎合并糖尿病患者。我们对他们进行了牙科检查,评估了他们的血糖水平,收集了他们的唾液,并检测了唾液中 IL-17 和 Del-1 的水平。我们发现,与健康受试者相比,牙周炎患者和牙周炎合并糖尿病患者的 IL-17 水平较高,而 Del-1 水平较低。与牙周炎患者相比,牙周炎合并糖尿病患者的IL-17水平较高,而Del-1水平较低。唾液中的IL-17和Del-1水平均与牙科检查结果和血糖水平相关,唾液中的IL-17和Del-1呈反比关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of salivary interleukin-17 and developmental endothelial locus-1 in patients with periodontitis with and without type 2 diabetes mellitus.

Background: Bidirectional positive relationship between periodontitis and type 2 diabetes mellitus (T2DM) has been recognized, interleukin 17 (IL-17) and developmental endothelial locus-1 (Del-1) are proposed to play roles in periodontitis and T2DM. This study aims to investigate the association of IL-17 and Del-1 in patients with periodontitis with and without T2DM by measuring their salivary levels.

Methods: A total of 80 participants were enrolled in a cross-sectional study and divided into healthy (H, n = 27), periodontitis (P, n = 29) and periodontitis with diabetes (PDM, n = 24) groups based on their periodontal and diabetic examination results. Periodontal parameters (plaque index [PI], bleeding on probing [BOP], probing depth [PD], and clinical attachment level [CAL]) as well as diabetic parameters (fasting plasma glucose [FG] and glycated hemoglobin [HbA1c]) were documented and unstimulated saliva was collected. Salivary IL-1β, active-matrix metalloproteinase-8 (aMMP-8), tumor necrosis factor-α (TNF-α), IL-6, IL-8, IL-17, and Del-1 were determined through enzyme-linked immunosorbent assay (ELISA) and their relationships with periodontal and diabetic parameters were examined.

Results: The periodontitis and periodontitis with diabetes groups showed significantly higher levels of IL-17 and lower levels of Del-1 compared with healthy group. The periodontitis with diabetes group exhibited higher levels of IL-17 and lower levels of Del-1 compared with the periodontitis group. After correlation analysis, there were significant correlations between salivary IL-17 and Del-1 and clinical parameters, IL-17 and Del-1 were correlated with PD (r = 0.36, -0.39, p < 0.01), CAL (r = 0.40, -0.42, p < 0.01) and BOP (r = 0.35, -0.37, p < 0.01), they were correlated with FG (r = 0.26, -0.25, p < 0.05) and HbA1c (r = 0.28, -0.40, p < 0.05). Positive relationships were observed between IL-17 and IL-1β and between IL-17 and aMMP-8 (r = 0.80, 0.77, p < 0.01), while Del-1 exhibited negative correlations with IL-1β and aMMP-8 (r = 0.59, 0.69 p < 0.01). Comparison between IL-17 and Del-1 confirmed an inverse relationship (r = -0.71, p < 0.01). Salivary Del-1 levels in the older group were lower compared with young group across the H, P and PDM groups, although these differences were not statistically significant (p > 0.05).

Conclusions: Salivary IL-17 and Del-1 levels in the periodontitis with diabetes group showed significant changes compared with the periodontitis group, they exhibited an inverse relationship and were both correlated with periodontal parameters (PD, CAL, and BOP) and diabetic parameters (FG and HbA1c).

Plain language summary: Periodontitis and type 2 diabetes mellitus (T2DM) are two common diseases all over the world, some inflammatory mediators (interleukin 17 [IL-17] and developmental endothelial locus-1 [Del-1]) regulate neutrophil production, recruitment and clearance when the body becomes infected and believed to be involved in the progress of diseases of periodontitis and diabetes. In this study, we enrolled healthy subjects, patients with periodontitis, patients with periodontitis and diabetes. We performed dental examinations and evaluated their blood glucose levels, collected their saliva, and detected IL-17 and Del-1 levels in their saliva. We found both patients with periodontitis and patients with periodontitis and diabetes showed higher IL-17 levels and lower Del-1 levels compared with healthy subjects. Patients with periodontitis and diabetes showed higher IL-17 levels and lower Del-1 levels compared with patients with periodontitis. Salivary IL-17 and Del-1 levels were both correlated with dental examination results and blood glucose levels, and salivary IL-17 and Del-1 displayed an inverse relationship.

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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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