禽传染性支气管炎病毒:埃塞俄比亚西南部鸡群的分子检测。

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-09-14 eCollection Date: 2024-01-01 DOI:10.1155/2024/6979448
Bezina Arega Emeru, Haregawi Tesfaye Desta
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引用次数: 0

摘要

传染性支气管炎病毒(IBV)是全球家禽面临的一个重大威胁,但其在埃塞俄比亚的状况仍未得到充分研究。因此,本研究旨在检测埃塞俄比亚西南部的病毒及相关风险因素。2021 年 11 月至 2022 年 4 月期间,研究人员从吉马地区的吉马镇、Seqa Chekorsa 和 Tiro Afeta 县有症状的鸡中有目的地采集了 90 份口咽拭子样本,采用 RT-PCR 技术检测 IBV 病毒。同时进行问卷调查以评估风险因素。提取总 RNA,进行反转录聚合酶链反应(RT-PCR),并在紫外光下观察产物。IBV的总比例为16.6%(15/90)。未发现任何动物风险因素与病毒检测之间存在统计学关联(P=0.57、0.586 和 1)。不过,与雌禽、本地品种和幼禽相比,雄禽、外来品种和成年禽感染病毒的比例较高。同样,所有管理风险因素对病毒检测的影响均无明显差异(P=0.25、0.09、0.088 和 0.726)。然而,胴体处理不当(OR = 0.43,95% CI:0.13-1.4)、缺乏兽医服务(OR = 2.7,95% CI:0.8-8.3)和野鸟/啮齿动物的存在(OR = 4.4,95% CI:0.88-22.3)与 IBV 风险增加有关,但与喂食器/饮水器的清洁无关(OR = 1.1,95% CI:0.2-4.8)。这些发现强调了加强生物安全措施和进一步研究的必要性,以便在埃塞俄比亚实施明智的 IBV 控制策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Avian Infectious Bronchitis Virus: Molecular Detection in Southwestern Ethiopia Chickens.

Infectious bronchitis virus (IBV) is a significant threat to poultry worldwide, but its status in Ethiopia remains understudied. Thus, this study aimed to detect the virus and associated risk factors in South West Ethiopia. Ninety oropharyngeal swab samples were purposively collected from symptomatic chickens located in Jimma town, Seqa Chekorsa, and Tiro Afeta woredas of the Jimma zone between November 2021 and April 2022 to detect IBV virus by using RT-PCR. A side-by-side questionnaire was administered to assess risk factors. Total RNA was extracted, reverse transcription polymerase chain reaction (RT-PCR) was conducted, and products were visualized under UV light. The overall proportion of IBV was 16.6% (15/90). No statistical association was observed between any of the animal risk factors and the detection of the virus (P=0.57, 0.586, and 1). However, the proportion of birds infected by the virus was higher in males, exotic breeds, and adults compared to females, local breeds, and young birds. Similarly, none of the management risk factors had a significantly different effect on virus detection (P=0.25, 0.09, 0.088, and 0.726). However, improper carcass disposal (OR = 0.43, 95% CI: 0.13-1.4), lack of veterinary services (OR = 2.7, 95% CI: 0.8-8.3), and the presence of wild birds/rodents (OR = 4.4, 95% CI: 0.88-22.3) were associated with increased IBV risk but not cleaning of feeders/drinkers (OR = 1.1, 95% CI: 0.2-4.8). These findings underscore the need for enhanced biosecurity practices and further research to implement informed IBV control strategies in Ethiopia.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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