研究由牙髓干细胞分化而成的成骨细胞产生的炎症细胞因子 TNF-α 和 IL-8 的水平。

IF 1.5 Q4 CELL BIOLOGY
American journal of stem cells Pub Date : 2024-08-25 eCollection Date: 2024-01-01 DOI:10.62347/CBMW4366
Sahar Khastar, Mandana Sattari
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引用次数: 0

摘要

背景:在临床应用中使用牙髓干细胞(DPSCs)代替骨髓干细胞是一种非常有前途的方法,能够极大地改变医疗的未来。如果进一步的研究证明牙髓干细胞及其分化出的细胞不会刺激免疫系统,那么这些细胞就能更可靠地用于治疗自身免疫性疾病:本研究采用 qRT-PCR 技术检测了在不含炎症刺激物的培养基中分离出的 DPSCs 及其分化出的成骨细胞的 TLR3 和 TLR4 基因表达以及包括 TNF-α 和 IL-8 在内的炎症细胞因子,并通过 ELISA 方法测定了这两种细胞产生的炎症细胞因子 IL-8 和 TNF-α 的浓度:结果表明,与 DPSCs 相比,炎性细胞因子 IL-8 和 TNF-α 在分化成骨细胞中的表达水平有显著差异。然而,在 TLR-4 的表达上,两组之间没有观察到明显差异。研究发现,TNF-α表达水平的增加与IL-8表达的增加直接相关。成骨细胞中细胞因子 TNF-α 的浓度明显高于 DPSCs 中 IL-8 的浓度:结论:与 DPSCs 相比,成骨细胞首先导致炎症反应。结论:与 DPSCs 相比,成骨细胞首先会导致炎症反应,这些反应会随着时间的推移而减少。结论:与 DPSCs 相比,成骨细胞首先会导致炎症反应,但随着时间的推移,这些反应会逐渐减弱。然而,DPSCs 保留了其免疫调节特性,不会出现炎症反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Examining the level of inflammatory cytokines TNF-α and IL-8 produced by osteoblasts differentiated from dental pulp stem cells.

Background: The use of dental pulp stem cells (DPSCs) in clinical applications instead of bone marrow stem cells is a very promising method capable of significantly changing the future of medical treatment. If further studies prove that DPSCs and the cells differentiated from them do not stimulate the immune system, these cells can be used more reliably in treatment of autoimmune diseases.

Methods: In this research, we examined the isolated DPSCs and differentiated osteoblasts from them in medium without inflammatory stimulants in terms of TLR3 and TLR4 gene expression and inflammatory cytokines, including TNF-α and IL-8 using qRT-PCR, and measured the concentration of inflammatory cytokines IL-8 and TNF-α produced by these two types of cells through ELISA.

Results: The obtained results showed that the expression level of inflammatory cytokines IL-8 and TNF-α in differentiated osteoblasts is significantly different as compared with DPSCs. However, no significant difference was observed in TLR-4 expression between two groups. An increase in TNF-α expression level was found to directly correlate with an increase in the expression of IL-8. The concentration of cytokine TNF-α in osteoblasts was significantly higher than that of IL-8 in DPSCs.

Conclusion: In comparison to DPSCs, osteoblast cells first lead to inflammatory responses. These responses reduce overtime. However, DPSCs retain their immunomodulatory properties and do not show inflammatory responses.

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