投射到上丘的GABA能视网膜神经节细胞介导卢明诱发的飞行反应

IF 5.9 2区 医学 Q1 NEUROSCIENCES
Man Yuan, Gao Tan, Danrui Cai, Xue Luo, Kejiong Shen, Qinqin Deng, Xinlan Lei, Wen-Bo Zeng, Min-Hua Luo, Lu Huang, Chaoran Ren, Yin Shen
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引用次数: 0

摘要

对接近的捕食者的隐约刺激诱发的飞行反应是大多数动物的一种防御性行为。然而,如何在视网膜中检测到隐现的刺激并将其传递到大脑仍不清楚。在这里,我们报告了一组投射到上丘脑(SC)的GABA能视网膜神经节细胞(RGCs)将掠食信号从视网膜传递到大脑,通过释放GABA来介导掠食诱发的飞行行为。GAD2-Cre 和 vGAT-Cre 转基因小鼠与 Cre 激活的前向或逆向示踪病毒结合使用,绘制了特定 GABA 能 RGC 回路的输入图。光遗传学技术被用来评估SC投射的GABA能RGCs(spgRGCs)在SC中的功能。FDIO-DTA(Flp依赖性双叶倒置开放阅读框-白喉毒素)与FLP(氟苯尼考、林可霉素& 泼尼松龙)相结合的方法被用来消减或沉默spgRGCs。在小鼠视网膜中,GABA能RGC投射到不同的脑区,包括SC。ScpgRGCs与副发光素阳性的SC神经元单突触连接,已知副发光素阳性的SC神经元是隐约诱发的飞行反应所必需的。光遗传激活spgRGCs会触发GABA介导的SC神经元抑制。spgRGCs的消融或沉默会影响隐约诱发的飞行反应,但不会影响图像形成功能。我们的研究揭示了spgRGCs通过GABA调节SC神经元来控制掠影诱发的飞行反应,为先天防御行为的调控提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

GABAergic Retinal Ganglion Cells Projecting to the Superior Colliculus Mediate the Looming-Evoked Flight Response

GABAergic Retinal Ganglion Cells Projecting to the Superior Colliculus Mediate the Looming-Evoked Flight Response

The looming stimulus-evoked flight response to approaching predators is a defensive behavior in most animals. However, how looming stimuli are detected in the retina and transmitted to the brain remains unclear. Here, we report that a group of GABAergic retinal ganglion cells (RGCs) projecting to the superior colliculus (SC) transmit looming signals from the retina to the brain, mediating the looming-evoked flight behavior by releasing GABA. GAD2-Cre and vGAT-Cre transgenic mice were used in combination with Cre-activated anterograde or retrograde tracer viruses to map the inputs to specific GABAergic RGC circuits. Optogenetic technology was used to assess the function of SC-projecting GABAergic RGCs (scpgRGCs) in the SC. FDIO-DTA (Flp-dependent Double-Floxed Inverted Open reading frame-Diphtheria toxin) combined with the FLP (Florfenicol, Lincomycin & Prednisolone) approach was used to ablate or silence scpgRGCs. In the mouse retina, GABAergic RGCs project to different brain areas, including the SC. ScpgRGCs are monosynaptically connected to parvalbumin-positive SC neurons known to be required for the looming-evoked flight response. Optogenetic activation of scpgRGCs triggers GABA-mediated inhibition in SC neurons. Ablation or silencing of scpgRGCs compromises looming-evoked flight responses without affecting image-forming functions. Our study reveals that scpgRGCs control the looming-evoked flight response by regulating SC neurons via GABA, providing novel insight into the regulation of innate defensive behaviors.

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来源期刊
Neuroscience bulletin
Neuroscience bulletin NEUROSCIENCES-
CiteScore
7.20
自引率
16.10%
发文量
163
审稿时长
6-12 weeks
期刊介绍: Neuroscience Bulletin (NB), the official journal of the Chinese Neuroscience Society, is published monthly by Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) and Springer. NB aims to publish research advances in the field of neuroscience and promote exchange of scientific ideas within the community. The journal publishes original papers on various topics in neuroscience and focuses on potential disease implications on the nervous system. NB welcomes research contributions on molecular, cellular, or developmental neuroscience using multidisciplinary approaches and functional strategies. We feature full-length original articles, reviews, methods, letters to the editor, insights, and research highlights. As the official journal of the Chinese Neuroscience Society, which currently has more than 12,000 members in China, NB is devoted to facilitating communications between Chinese neuroscientists and their international colleagues. The journal is recognized as the most influential publication in neuroscience research in China.
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