胎盘组织处理的技术考虑因素及其对全基因组 DNA 甲基化分析的影响

IF 4.8 2区医学 Q1 GENETICS & HEREDITY

Clinical Epigenetics Pub Date : 2024-09-16 DOI:10.1186/s13148-024-01741-8

Kristen M. Kocher, Julius Ngwa, Kushal J. Kapse, Surajit Bhattacharya, Christopher Rossi, Emmanuele Delot, Adre duPlessis, Catherine Limperopoulos, Nickie Andescavage

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引用次数: 0

摘要

为了评估产后处理对胎盘DNA甲基化的影响，我们将速冻胎盘组织的阵列数据与全氟浸泡胎盘组织和福尔马林固定石蜡包埋胎盘组织进行了比较。我们观察到，与未经处理的组织相比，浸泡在全氟碳化物中的组织没有显示出明显的DNA甲基化差异，而福尔马林处理则改变了DNA甲基化阵列平台上产生的数据的质量和可靠性。胎盘 DNA 甲基化有助于研究影响胎儿环境和发育的基因与环境之间的相互作用。我们的研究强调，在评估和解释表观遗传学研究时必须考虑胎盘后处理技术。

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Technical considerations for placental tissue processing and the subsequent impact on genome-wide DNA methylation analysis

To assess the impact of postnatal processing on placental DNA methylation, array data from flash-frozen placental tissue was compared to perfluorocarbon-immersed and formalin-fixed paraffin-embedded placental tissue. We observed that tissue exposed to perfluorocarbon showed no significant DNA methylation differences when compared to unprocessed tissue, while formalin processing altered the quality and reliability of the data produced on the DNA methylation array platform. Placental DNA methylation allows for the study of gene–environment interactions that influence the fetal environment and development. Our study highlights that placental post-processing techniques must be considered in the evaluation and interpretation of epigenetic studies.

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来源期刊

Clinical Epigenetics ONCOLOGY-

自引率

5.30%

发文量

150

期刊介绍： Clinical Epigenetics, the official journal of the Clinical Epigenetics Society, is an open access, peer-reviewed journal that encompasses all aspects of epigenetic principles and mechanisms in relation to human disease, diagnosis and therapy. Clinical trials and research in disease model organisms are particularly welcome.