{"title":"通过数字图像比色法估算异烟肼：绿色化学与智能手机技术的融合","authors":"Suvarna Yenduri, K. Naga Prashant","doi":"10.1186/s43094-024-00703-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>This study proposes a simple digital image colorimetric method using a smartphone for the quantitative analysis of isoniazid in pharmaceutical medications. The analytical methodology employed in this study involved the utilization of the reaction between isoniazid and FC reagent under alkaline conditions. The chemical reaction led to the creation of a complex with a blue-gray color, exhibiting a peak absorption at a wavelength of 760 nm. An Android application was employed to perform a smartphone-based determination based on separating the captured color into different color channels such as red, blue, green, etc. The experimental procedure involved the utilization of three smartphones for the determination, which was subsequently followed by a comparative analysis of the results obtained from these devices using spectrophotometric measurements.</p><h3>Results</h3><p>The B channel had the highest level of optimization in terms of analytical parameters. The limits of detection (LOD) were 0.586, 2.478, and 1.396 µg/ml for S1, S2, and S3, respectively, and for the spectrophotometer, it was found to be 0.416 µg/ml. Similarly, LOQs were determined as 1.673, 7.511, 4.232, and 1.302 µg/ml. A %RSD of 1.3 for precision and an LOD of 0.013 g dm<sup>−3</sup> were obtained for the spectroscopic method. The % recovery in the accuracy study was found to be 99.69, 101.804, 109.28, and 100.13 for S1, S2, S3, and spectrophotometer, respectively.</p><h3>Conclusion</h3><p>The colorimetric results from smartphone application are similar to those from spectrophotometric analyses.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00703-4","citationCount":"0","resultStr":"{\"title\":\"Estimation of isoniazid by digital image colorimetry: an integration of green chemistry and smartphone technology\",\"authors\":\"Suvarna Yenduri, K. Naga Prashant\",\"doi\":\"10.1186/s43094-024-00703-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>This study proposes a simple digital image colorimetric method using a smartphone for the quantitative analysis of isoniazid in pharmaceutical medications. The analytical methodology employed in this study involved the utilization of the reaction between isoniazid and FC reagent under alkaline conditions. The chemical reaction led to the creation of a complex with a blue-gray color, exhibiting a peak absorption at a wavelength of 760 nm. An Android application was employed to perform a smartphone-based determination based on separating the captured color into different color channels such as red, blue, green, etc. The experimental procedure involved the utilization of three smartphones for the determination, which was subsequently followed by a comparative analysis of the results obtained from these devices using spectrophotometric measurements.</p><h3>Results</h3><p>The B channel had the highest level of optimization in terms of analytical parameters. The limits of detection (LOD) were 0.586, 2.478, and 1.396 µg/ml for S1, S2, and S3, respectively, and for the spectrophotometer, it was found to be 0.416 µg/ml. Similarly, LOQs were determined as 1.673, 7.511, 4.232, and 1.302 µg/ml. A %RSD of 1.3 for precision and an LOD of 0.013 g dm<sup>−3</sup> were obtained for the spectroscopic method. The % recovery in the accuracy study was found to be 99.69, 101.804, 109.28, and 100.13 for S1, S2, S3, and spectrophotometer, respectively.</p><h3>Conclusion</h3><p>The colorimetric results from smartphone application are similar to those from spectrophotometric analyses.</p></div>\",\"PeriodicalId\":577,\"journal\":{\"name\":\"Future Journal of Pharmaceutical Sciences\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00703-4\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s43094-024-00703-4\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s43094-024-00703-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Estimation of isoniazid by digital image colorimetry: an integration of green chemistry and smartphone technology
Background
This study proposes a simple digital image colorimetric method using a smartphone for the quantitative analysis of isoniazid in pharmaceutical medications. The analytical methodology employed in this study involved the utilization of the reaction between isoniazid and FC reagent under alkaline conditions. The chemical reaction led to the creation of a complex with a blue-gray color, exhibiting a peak absorption at a wavelength of 760 nm. An Android application was employed to perform a smartphone-based determination based on separating the captured color into different color channels such as red, blue, green, etc. The experimental procedure involved the utilization of three smartphones for the determination, which was subsequently followed by a comparative analysis of the results obtained from these devices using spectrophotometric measurements.
Results
The B channel had the highest level of optimization in terms of analytical parameters. The limits of detection (LOD) were 0.586, 2.478, and 1.396 µg/ml for S1, S2, and S3, respectively, and for the spectrophotometer, it was found to be 0.416 µg/ml. Similarly, LOQs were determined as 1.673, 7.511, 4.232, and 1.302 µg/ml. A %RSD of 1.3 for precision and an LOD of 0.013 g dm−3 were obtained for the spectroscopic method. The % recovery in the accuracy study was found to be 99.69, 101.804, 109.28, and 100.13 for S1, S2, S3, and spectrophotometer, respectively.
Conclusion
The colorimetric results from smartphone application are similar to those from spectrophotometric analyses.
期刊介绍：
Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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