环甲基铱(III)与取代的苯并咪唑配合物：溶酶体内 pH 值定向的细胞器特异性定位

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

ChemBioChem Pub Date : 2024-09-17 DOI:10.1002/cbic.202400597

Subhra Sil, Afaq Hussain, Jayasri Das Sarma, Parna Gupta

{"title":"环甲基铱(III)与取代的苯并咪唑配合物：溶酶体内 pH 值定向的细胞器特异性定位","authors":"Subhra Sil, Afaq Hussain, Jayasri Das Sarma, Parna Gupta","doi":"10.1002/cbic.202400597","DOIUrl":null,"url":null,"abstract":"We report the synthesis and pH dependent emission spectral behaviour of four emissive iridium(III) complexes (Ir1 – Ir4) with two isomeric pairs of bis‐trifluoromethyl appended benzimidazole ligands. The imidazolyl hydrogen(N‐H) has been replaced by phenyl groups (N‐Ph) in two ligands to understand the impact of hydrogen bonding on the photophysical properties of the complexes and it indeed plays interesting role in the charge‐transfer dynamics. The pH dependent electronic spectral change is observed for two of the complexes. The enhancement of emission intensity is observed at different wavelength for pH < 7 and pH > 7 for Ir1 and Ir3. The emission sensing of biogenic amines with pka values ranging from 5.80 – 9.74 is reported along with cellular imaging. The complex Ir1 specifically localizes within lysosome (pH = 4.5 – 5) and thus image this organelle with great precision. The detail electronic spectra and electrochemical behaviour were reported here along with TDDFT results.","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cyclometalated Iridium(III) Complex with Substituted Benzimidazole: pH Directed Organelle‐specific Localization within Lysosome\",\"authors\":\"Subhra Sil, Afaq Hussain, Jayasri Das Sarma, Parna Gupta\",\"doi\":\"10.1002/cbic.202400597\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We report the synthesis and pH dependent emission spectral behaviour of four emissive iridium(III) complexes (Ir1 – Ir4) with two isomeric pairs of bis‐trifluoromethyl appended benzimidazole ligands. The imidazolyl hydrogen(N‐H) has been replaced by phenyl groups (N‐Ph) in two ligands to understand the impact of hydrogen bonding on the photophysical properties of the complexes and it indeed plays interesting role in the charge‐transfer dynamics. The pH dependent electronic spectral change is observed for two of the complexes. The enhancement of emission intensity is observed at different wavelength for pH < 7 and pH > 7 for Ir1 and Ir3. The emission sensing of biogenic amines with pka values ranging from 5.80 – 9.74 is reported along with cellular imaging. The complex Ir1 specifically localizes within lysosome (pH = 4.5 – 5) and thus image this organelle with great precision. The detail electronic spectra and electrochemical behaviour were reported here along with TDDFT results.\",\"PeriodicalId\":140,\"journal\":{\"name\":\"ChemBioChem\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemBioChem\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/cbic.202400597\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/cbic.202400597","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

我们报告了四种发射性铱（III）配合物（Ir1 - Ir4）的合成和与 pH 值相关的发射光谱行为，这四种配合物具有两对异构的双三氟甲基附加苯并咪唑配体。两个配体中的咪唑氢（N-H）被苯基（N-Ph）取代，以了解氢键对配合物光物理性质的影响。在其中两种配合物中观察到了与 pH 值相关的电子光谱变化。Ir1 和 Ir3 在 pH < 7 和 pH > 7 的不同波长下，发射强度都有所增强。报告还介绍了对 pka 值在 5.80 - 9.74 之间的生物胺的发射传感以及细胞成像。复合物 Ir1 在溶酶体（pH = 4.5 - 5）内特异性定位，因此可以非常精确地对这一细胞器进行成像。本文报告了详细的电子光谱和电化学行为以及 TDDFT 结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Cyclometalated Iridium(III) Complex with Substituted Benzimidazole: pH Directed Organelle‐specific Localization within Lysosome

We report the synthesis and pH dependent emission spectral behaviour of four emissive iridium(III) complexes (Ir1 – Ir4) with two isomeric pairs of bis‐trifluoromethyl appended benzimidazole ligands. The imidazolyl hydrogen(N‐H) has been replaced by phenyl groups (N‐Ph) in two ligands to understand the impact of hydrogen bonding on the photophysical properties of the complexes and it indeed plays interesting role in the charge‐transfer dynamics. The pH dependent electronic spectral change is observed for two of the complexes. The enhancement of emission intensity is observed at different wavelength for pH < 7 and pH > 7 for Ir1 and Ir3. The emission sensing of biogenic amines with pka values ranging from 5.80 – 9.74 is reported along with cellular imaging. The complex Ir1 specifically localizes within lysosome (pH = 4.5 – 5) and thus image this organelle with great precision. The detail electronic spectra and electrochemical behaviour were reported here along with TDDFT results.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

ChemBioChem 生物-生化与分子生物学

CiteScore

6.10

自引率

3.10%

发文量

407

审稿时长

1 months

期刊介绍： ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).