白藜芦醇对分裂后期玻璃化猫科动物卵母细胞发育能力的影响

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Agnieszka Nowak , Joanna Kochan , Julia Gabryś , Barbara Kij-Mitka , Wojciech Witarski , Sylwia Prochowska , Wojciech Niżański
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引用次数: 0

摘要

本研究的目的是评估猫卵母细胞在体外成熟(IVM)、玻璃化和白藜芦醇温育后的存活率和发育潜力。在第一项实验中,用 0.2 μM、2 μM 或 20 μM 白藜芦醇孵育温育卵母细胞 2 小时。根据荧光素二乙酸酯和溴化乙锭染色法评估,用 0.2 μM 白藜芦醇处理的卵母细胞存活率最高(68.89%),而浓度越高,卵母细胞存活率越低。在第二个实验中,用三种浓度的白藜芦醇孵育 2 小时后,对温育卵母细胞进行人工授精。然后将推定的合子进行培养,并对其发育情况进行评估。用 0.2 μM 白藜芦醇孵育的卵母细胞裂殖率最高(88.34%),高于对照组(不含白藜芦醇(75%))。此外,该浓度的白藜芦醇还能提高囊胚形成率。卵母细胞玻璃化通常会导致胚胎发育潜能下降,这归因于冷冻引起的损伤,而低浓度白藜芦醇的使用则提高了这一过程的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of resveratrol on the developmental competence of feline oocytes vitrified at the metaphase II stage

The objective of this research was to assess the viability and developmental potential of feline oocytes following in vitro maturation (IVM), vitrification, and post-warming incubation with resveratrol. In the first experiment, warmed oocytes were incubated with 0.2 μM, 2 μM, or 20 μM resveratrol for 2 h. Oocytes treated with 0.2 μM resveratrol had the highest viability (68.89 %), as assessed by fluorescein diacetate and ethidium bromide staining, while higher concentrations were associated with diminished oocyte viability. In the second experiment, the warmed oocytes were inseminated following the 2-h incubation with the three concentrations of resveratrol. The presumptive zygotes were then maintained in culture and their development evaluated. The highest cleavage rate was observed when the oocytes had been incubated with 0.2 μM resveratrol (88.34 %), which was higher than for the control group (without resveratrol (75 %)). Moreover, this concentration of resveratrol also augmented the blastocyst formation rate. While the vitrification of oocytes often results in diminished developmental potential in the ensuing embryos, attributed to cryopreservation-induced injury, the utilization of low concentrations of resveratrol enhances the procedure's efficacy.

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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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