合成生物分子凝聚态工程的最新进展

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Li Wan, Juntao Ke, Yingying Zhu, Wenli Zhang, Wanmeng Mu
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引用次数: 0

摘要

生物分子凝聚体是活细胞内发现的有趣实体。这些结构具有通过相分离选择性地浓缩特定成分的能力,从而在多种细胞过程和新陈代谢活动的时空调控中发挥着至关重要的作用。迄今为止,已有大量研究致力于揭示凝聚物的分子特征、物理特性和细胞功能之间错综复杂的联系。这一集体努力为精心设计具有特定应用的定制凝集物铺平了道路。在这篇综述中,我们将全面考察凝结物形成的基础。接着,我们总结了凝聚态的物质状态,并深入探讨了具有可调相行为和物理性质的合成本征无序蛋白的设计。随后,我们回顾了合成生物分子凝聚态所展示的各种生物功能,包括基因调控、细胞行为、生化反应调控和内源蛋白质活性调控。最后,我们讨论了构建具有可调物理性质和定制细胞功能的合成凝聚态的未来挑战和机遇,这可能为开发具有适用于各种场景的独特功能的新型复杂凝聚态系统提供启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Recent advances in engineering synthetic biomolecular condensates

Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections between molecular features, physical properties, and cellular functions of condensates. This collective effort has paved the way for deliberate engineering of tailor-made condensates with specific applications. In this review, we comprehensively examine the underpinnings governing condensate formation. Next, we summarize the material states of condensates and delve into the design of synthetic intrinsically disordered proteins with tunable phase behaviors and physical properties. Subsequently, we review the diverse biological functions demonstrated by synthetic biomolecular condensates, encompassing gene regulation, cellular behaviors, modulation of biochemical reactions, and manipulation of endogenous protein activities. Lastly, we discuss future challenges and opportunities in constructing synthetic condensates with tunable physical properties and customized cellular functions, which may shed light on the development of new types of sophisticated condensate systems with distinct functions applicable to various scenarios.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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