由硼酸修饰的 PNA 及其配体组成的荧光复合物可检测细胞中的目标 RNA

IF 1.4 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Chemistry Letters Pub Date : 2024-08-24 DOI:10.1093/chemle/upae169

Koki Ishii, Yoshihide Hattori, Hajime Shigeto, Shohei Yamamura, Mizuki Kitamatsu

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引用次数: 0

摘要

我们开发了用硼酸（Boa）及其配体 2-(吡啶-2-基)苯酚（Pyp）修饰的肽核酸（PNA），作为荧光检测目标核酸的探针。Boa 和 Pyp 通过与 PNA 和目标物杂交复合，成功地显示出荧光。这种荧光 PNA 探针还成功地与细胞中的目标 RNA 发生反应。

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A fluorescent complex with PNAs modified by boronic acid and its ligand detects target RNA in cells

We have developed peptide nucleic acids (PNAs) modified with boronic acid (Boa) and its ligand 2-(pyridin-2-yl)phenol (Pyp) as a probe for fluorescence detection of a target nucleic acid. Boa and Pyp successfully showed fluorescence by complexing via hybridization with PNA and the target. This fluorescent PNA probe also successfully responded to the target RNA in cells.

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来源期刊

Chemistry Letters 化学-化学综合

CiteScore

3.00

自引率

6.20%

发文量

260

审稿时长

1.2 months

期刊介绍： Chemistry Letters covers the following topics: -Organic Chemistry- Physical Chemistry- Inorganic Chemistry- Analytical Chemistry- Materials Chemistry- Polymer Chemistry- Supramolecular Chemistry- Organometallic Chemistry- Coordination Chemistry- Biomolecular Chemistry- Natural Products and Medicinal Chemistry- Electrochemistry