卡瓦卡瓦(Piper excelsum)的抗炎作用:mRNA-miRNA综合方法

IF 3.5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Senilaite Tautuiaki, Jerusha Gojer, Ramya Jayaprakash, Pankaja Sharma, Chris Pook, Meika Foster, Jennifer Miles‐Chan, Richard Mithen, Farha Ramzan
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引用次数: 0

摘要

卡瓦卡瓦(Piper excelsum)是一种新西兰特有的药用植物,被新西兰毛利人广泛食用。卡瓦卡瓦(Kawakawa)中含有多种生物活性植物化学物质,这可能是其抗炎治疗特性的基础。不过,目前还没有对其抗炎作用进行人体研究。本研究分析了一组健康志愿者(n = 26;年龄;33.6 ± 1.9 岁;体重指数;22.5 ± 0.4 kg/m2)的随机对照饮食干预血液样本,探讨了与对照组相比,卡瓦卡瓦对餐后微核糖核酸(miRNA)丰度及其各自的基因和蛋白质靶标的影响。与对照组相比,hsa-miR-17-5p、-21-5p、-320a-5p、let-7g-5p、-16-5p、-122-5p和-144-3p在对卡瓦卡瓦的反应中上调,而hsa-miR-221-3p和-223-3p在对卡瓦卡瓦的反应中下调。硅学分析表明,miRNA 在多种炎症相关通路中富集,包括细胞凋亡、细胞因子信号转导、MAPK 信号转导和 MTOR 通路。此外,与对照组相比,摄入卡瓦卡瓦后,IL-8(p = .03)、IL-6(p = .01)和 PPAR-γ 的基因表达明显降低。而在 120 分钟内,血浆 IL-6 在卡瓦卡瓦组显示出明显增加。这些结果表明,卡瓦卡瓦能通过调节炎症信号通路中的 miRNAs 及其靶基因和蛋白质的表达来发挥抗炎作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti‐inflammatory effects of kawakawa (Piper excelsum): An integrative mRNA–miRNA approach
Kawakawa (Piper excelsum) is an endemic medicinal plant widely consumed by Māori in New Zealand. Presence of diverse biologically active phytochemicals in kawakawa may underpin its putative therapeutic anti‐inflammatory properties. However, no human studies on its anti‐inflammatory effects are yet undertaken. Blood samples from a randomized controlled dietary intervention exploring the impact of kawakawa compared to control on postprandial microRNAs (miRNA) abundances and their respective gene and protein targets in a cohort of healthy human volunteers (n = 26; Age; 33.6 ± 1.9 year and BMI; 22.5 ± 0.4 kg/m2) were analyzed. Postprandial levels of nine miRNAs showed differential abundances; hsa‐miR‐17‐5p, ‐21‐5p, ‐320a‐5p, let‐7g‐5p, ‐16‐5p, ‐122‐5p, and ‐144‐3p was upregulated while as hsa‐miR‐221‐3p and ‐223‐3p was downregulated in response to kawakawa compared to control. In silico analysis indicated enrichment of miRNAs in multiple inflammation‐related pathways, including apoptosis, cytokine signaling, MAPK signaling, and MTOR pathways. Furthermore, gene expression of IL‐8 (p = .03), IL‐6 (p = .01), and PPAR‐γ were significantly reduced following kawakawa intake compared to control. While as plasma IL‐6 showed a significant increase over 120 min in the kawakawa arm. These results highlight kawakawa to exert anti‐inflammatory effects by modulating the expression of miRNAs and their target genes and proteins in the inflammatory signaling pathways.
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来源期刊
Food Science & Nutrition
Food Science & Nutrition Agricultural and Biological Sciences-Food Science
CiteScore
7.40
自引率
5.10%
发文量
434
审稿时长
24 weeks
期刊介绍: Food Science & Nutrition is the peer-reviewed journal for rapid dissemination of research in all areas of food science and nutrition. The Journal will consider submissions of quality papers describing the results of fundamental and applied research related to all aspects of human food and nutrition, as well as interdisciplinary research that spans these two fields.
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