Huan Wang, Guoqing Duan, Huaxing Zhou, Yuting Hu, Amei Liu
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引用次数: 0
摘要
黄蝉(Cuora flavomarginata)是中国的濒危保护物种,也被世界自然保护联盟(IUCN)列为濒危物种。单核苷酸多态性(SNPs)是动物基因组多样性的重要组成部分,是进化遗传学和保护遗传学领域不可多得的遗传标记。本研究在全基因组重测序的基础上,共开发了 40 个新型 SNP 标记。对 56 只龟的 40 个 SNP 基因型的分析表明,观察到的杂合度和预期杂合度分别为 0.1786 至 0.6071 和 0.1641 至 0.5043。小等位基因频率为 0.1161 至 0.911,多态性信息含量为 0.252 至 0.455。在这些 SNP 中,经 Bonferroni 校正后,只有一个 SNP 显著偏离 Hardy-Weinberg 平衡(P < 0.01)。这些 SNP 标记可能是该濒危物种遗传管理和种群保护的重要工具。
Development and characterization of 40 SNP markers of endangered species Cuora flavomarginata by whole-genome resequencing
Cuora flavomarginata is an endangered and protected species in China as well as listed as endangered under the criteria of the IUCN. Single nucleotide polymorphisms (SNPs) are an important component of animal genomic diversity, and are invaluable as genetic markers in the fields of evolutionary and conservation genetics. In this study, a total of 40 novel SNP markers were developed based on whole-genome resequencing. The analysis of the 40 SNP genotypes in 56 turtles showed that the observed heterozygosity and expected heterozygosity ranged from 0.1786 to 0.6071 and 0.1641 to 0.5043, respectively. The minor allele frequency ranged from 0.1161 to 0.911, and the polymorphism information content ranged from 0.252 to 0.455. Among these SNPs, only one SNP was found to deviate from Hardy-Weinberg equilibrium significantly after Bonferroni correction (P < 0.01). These SNP markers could be a valuable tool for genetic management and population conservation in this endangered species.
期刊介绍:
Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.