四十二个多态简单序列重复标记的分离和特征描述

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2024-09-11 DOI:10.1007/s12686-024-01365-3

Laifu Luo, Liqin Ji, Congcong Wu, Wei Li, Xiaoli Liu, Xiaoyou Hong, Xinping Zhu, Yijun Ni, Jiansong Li, Chen Chen, Jiping Zhang

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引用次数: 0

摘要

为保护 "人字鳗"（Mastacembelus armatus）的自然资源，研究人员从其基因组序列中开发了42个多态性简单序列重复（SSR）标记，并对其进行了表征。在一个 35 个个体的自然种群中对这些 SSR 标记的多态性进行了评估。每个位点的等位基因数量从 2 个到 14 个不等。观察到的杂合度和预期杂合度分别为 0.031 至 0.813 和 0.091 至 0.852。经 Bonferroni 校正后，三个 SSR 位点明显偏离 Hardy-Weinberg 平衡。这些位点首次得到了表征，将有助于M. armatus的遗传保护和标记辅助育种。

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Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus (Symbranchiformes: Mastacembelidae)

A total of 42 polymorphic simple sequence repeat (SSR) markers were developed and characterized from genome sequences for the “zig-zag eel”, Mastacembelus armatus, to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen. The observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci have been characterized for the first time and will be useful for genetic conservation and marker-assisted breeding of M. armatus.

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.