在公鸡精液扩展剂中添加荨麻水提取物以实现低温长时间保存

IF 1.2 4区 生物学 Q4 CELL BIOLOGY
Sina Naderi,Amjad Farzinpour,Asaad Vaziry,Abbas Farshad
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引用次数: 0

摘要

精子膜磷脂的过氧化是导致禽类精液保存过程中发生不可逆变化的主要原因。针对这一问题,本研究旨在评估荨麻提取物在长期储存期间保护禽类精子的潜力。本研究采用气相色谱-质谱技术来评估从荨麻的气生部分和根部提取的水提取物和乙醇提取物中的生物活性化合物。每周两次从 16 只公鸡身上采集精液样本,并在含有不同浓度(0、0.5 和 1 mg/100 mL)各种提取物的莱克扩展液中进行稀释。随后,将扩展后的精液样本冷却并储存在 5°C 下,并在储存 0、12、24 和 36 小时后对精子质量参数进行评估。实验数据清楚地表明,在精液稀释液中添加荨麻根水提取物,尤其是浓度为 0.5 毫克/100 毫升时,可显著改善精子的各种质量参数。值得注意的是,在体外储存长达 36 小时的公鸡精液中,精子的总运动能力和渐进运动能力、存活率、受精能力、膜完整性、顶体膜完整性都有所提高,丙二醛的生成也有所减少。有趣的是,本研究发现,荨麻不同部位的水提取物的有益作用不仅得到了传统人工方法的支持,而且还得到了计算机辅助精子分析系统的支持。这种双重确认进一步强调了水提取物在精液冷却保存过程中对精子各种性状的积极影响。总之,本研究强调了荨麻提取物,尤其是浓度为 0.5 mg/100 mL 的荨麻根水提取物在长期储存期间保护禽类精子的潜力。各种精子质量参数的明显改善以及人工和计算机辅助分析方法对结果的验证,为荨麻提取物在禽类育种计划和人工授精实践中的应用提供了有力的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Supplementation of Rooster Semen Extender with Aqueous Extract of Urtica dioica for a Long Time Preservation by Low Temperature.
The peroxidation of spermatozoa membrane phospholipids is a primary cause of irreversible changes in the preservation of avian semen. To address this issue, the objective of the present study was to assess the potential of Urtica dioica extracts in protecting avian spermatozoa during prolonged storage. Gas chromatography-mass spectroscopic techniques were employed to evaluate the bioactive compounds present in the aqueous and ethanolic extracts obtained from the aerial parts and roots of U. dioica. Semen samples were collected from 16 roosters twice a week and were diluted in Lake's extender containing different concentrations (0, 0.5, and 1 mg/100 mL) of the various extracts. Subsequently, the extended semen samples were cooled and stored at 5°C, and the sperm quality parameters were assessed at 0, 12, 24, and 36 hours of storage. The data from this experiment clearly demonstrate that the addition of nettle root aqueous extracts to the semen diluent, especially at a concentration of 0.5 mg/100 mL, resulted in a significant improvement in various sperm quality parameters. Notably, there were enhancements in total and progressive sperm motilities, viability, fertility, membrane integrity, acrosomal membrane integrity, and a reduction in malondialdehyde production in rooster semen stored in vitro for up to 36 hours. Interestingly, the present study reveals that the beneficial effects of the aqueous extracts from different parts of the nettle were supported not only by the conventional manual method but also by the computer-assisted sperm analysis system. This dual confirmation further emphasizes the positive impact of the aqueous extract on various sperm traits during cooled semen preservation. In conclusion, this study highlights the potential of U. dioica extracts, particularly the aqueous extract from nettle roots at a concentration of 0.5 mg/100 mL, in safeguarding avian spermatozoa during prolonged storage. The significant improvements in various sperm quality parameters and the validation of results through both manual and computer-assisted analysis methods provide strong evidence for the application of U. dioica extracts in avian breeding programs and artificial insemination practices.
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来源期刊
Biopreservation and Biobanking
Biopreservation and Biobanking CELL BIOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.30
自引率
12.50%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.
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