优化噬菌体疗法治疗难治的肌肉骨骼感染:从工作台到床边的视角

IF 4.6 2区 医学 Q2 IMMUNOLOGY
Laura Bessems, Baixing Chen, Saartje Uyttebroek, David Devolder, Cédric Lood, Stefaan Verwimp, Paul De Munter, Yves Debaveye, Melissa Depypere, Isabel Spriet, Laura Van Gerven, Lieven Dupont, Jeroen Wagemans, Vera van Noort, Rob Lavigne, Willem-Jan Metsemakers, Jolien Onsea
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引用次数: 0

摘要

鉴于抗菌药耐药性的威胁日益严重,科学家们正在紧急寻求辅助抗菌策略,如噬菌体疗法(PT)。然而,尽管我们中心在治疗肌肉骨骼感染方面取得了可喜的成果,但仍存在重要的知识空白。因此,我们开展了一项前瞻性观察研究(PHAGEFORCE),并采用多学科方法来实现和优化标准化治疗指南。在我们中心,PT 由一个多学科工作组严格控制和监测。每次噬菌体治疗都遵循相同的路径,以确保标准化和数据质量。在 PHAGEFORCE 框架内,我们建立了一个测试平台,以深入了解 PT 的安全性和有效性、生物分布、噬菌体动力学以及噬菌体与细菌之间的分子相互作用。通过收集引流液来确定噬菌体滴度和细菌负荷。此外,还通过基因组测序对所有细菌分离物进行全面鉴定,以监测噬菌体抗药性的出现。我们在此介绍一种标准化的从工作台到床边的方案,以便更深入地了解肌肉骨骼感染 PT 的动力学和动态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of bacteriophage therapy for difficult-to-treat musculoskeletal infections: a bench-to-bedside perspective
Given the increasing threat of antimicrobial resistance, scientists are urgently seeking adjunct antimicrobial strategies, such as phage therapy (PT). However, despite promising results for the treatment of musculoskeletal infections in our center, crucial knowledge gaps remain. Therefore, a prospective observational study (PHAGEFORCE) and a multidisciplinary approach was set up to achieve and optimize standardized treatment guidelines. At our center, PT is strictly controlled and monitored by a multidisciplinary taskforce. Each phage treatment follows the same pathway to ensure standardization and data quality. Within the PHAGEFORCE framework, we established a testing platform to gain insight in the safety and efficacy of PT, biodistribution, phage kinetics and the molecular interaction between phages and bacteria. The draining fluid is collected to determine the phage titer and bacterial load. In addition, all bacterial isolates are fully characterized by genome sequencing to monitor the emergence of phage resistance. We hereby present a standardized bench-to-bedside protocol to gain more insight in the kinetics and dynamics of PT for musculoskeletal infections.
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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
1817
审稿时长
14 weeks
期刊介绍: Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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