评估酵母菌分泌的具有潜在皮肤功效的生物技术活性肽

Elisabete Muchagato Maurício, Patrícia Branco, Ana Luiza Barros Araújo, Catarina Roma-Rodrigues, Katelene Lima, Maria Paula Duarte, Alexandra R. Fernandes, Helena Albergaria
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引用次数: 0

摘要

生物活性肽具有抗菌、消炎、抗氧化、抗胶原酶(ACE)作用以及伤口愈合特性,因此越来越受到化妆品行业的关注,也越来越适合用于化妆品配方。使用肉汤微稀释和挑战试验评估了乙醇红酵母菌分泌的肽(2-10 kDa)对皮肤病原体的抗菌活性。使用胶原酶活性比色法评估 ACE,通过分光光度法监测硝基四唑氯化蓝(NBT)还原来评估抗氧化活性,并通过量化暴露于脂多糖(LPS)的真皮成纤维细胞中的 TNF-α mRNA 来评估抗炎作用。伤口愈合试验涉及人成纤维细胞、内皮细胞和真皮角质细胞。肽(2-10 kDa)对 10 种皮肤病原体具有抗菌活性,最低抑制浓度(MIC)从金黄色葡萄球菌的 125 µg/mL 到白色念珠菌和化脓性链球菌的 1000 µg/mL 不等。在挑战试验中,除巴西曲霉菌外,肽的 MIC 值都能显著减少微生物数量,符合 ISO 11930:2019 标准。多肽与 MicrocareⓇ SB 的结合显示出协同作用,尤其是对白僵菌和巴西曲霉的作用。在体外,250 微克/毫升和 1000 微克/毫升的肽对胶原酶活性的抑制率分别为 41.8% 和 94.5%,并显示出抗氧化能力。与肽预孵育可降低成纤维细胞中 TNF-α 的表达,表明其具有抗炎作用。多肽没有显示出促进或抑制内皮细胞血管生成的作用,但能在伤口愈合过程的最后阶段减轻纤维化、疤痕形成和慢性炎症。这些肽具有抗菌、抗氧化、ACE和抗炎特性,突出了它们作为护肤品中多功能生物活性成分的潜力,值得进一步优化和探索其在化妆品中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefits
Biotechnological active peptides are gaining interest in the cosmetics industry due to their antimicrobial, anti-inflammatory, antioxidant, and anti-collagenase (ACE) effects, as well as wound healing properties, making them suitable for cosmetic formulations. The antimicrobial activity of peptides (2–10 kDa) secreted by Saccharomyces cerevisiae Ethanol-Red was evaluated against dermal pathogens using broth microdilution and challenge tests. ACE was assessed using a collagenase activity colorimetric assay, antioxidant activity via spectrophotometric monitoring of nitrotetrazolium blue chloride (NBT) reduction, and anti-inflammatory effects by quantifying TNF-α mRNA in lipopolysaccharides (LPS)-exposed dermal fibroblasts. Wound healing assays involved human fibroblasts, endothelial cells, and dermal keratinocytes. The peptides (2–10 kDa) exhibited antimicrobial activity against 10 dermal pathogens, with the Minimum Inhibitory Concentrations (MICs) ranging from 125 µg/mL for Staphylococcus aureus to 1000 µg/mL for Candida albicans and Streptococcus pyogenes. In the challenge test, peptides at their MICs reduced microbial counts significantly, fulfilling ISO 11930:2019 standards, except against Aspergillus brasiliensis. The peptides combined with MicrocareⓇ SB showed synergy, particularly against C. albicans and A. brasilensis. In vitro, the peptides inhibited collagenase activity by 41.8% and 94.5% at 250 and 1000 µg/mL, respectively, and demonstrated antioxidant capacity. Pre-incubation with peptides decreased TNF-α expression in fibroblasts, indicating anti-inflammatory effects. The peptides do not show to promote or inhibit the angiogenesis of endothelial cells, but are able to attenuate fibrosis, scar formation, and chronic inflammation during the final phases of the wound healing process. The peptides showed antimicrobial, antioxidant, ACE, and anti-inflammatory properties, highlighting their potential as multifunctional bioactive ingredients in skincare, warranting further optimization and exploration in cosmetic applications.
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