6-苄基氨基嘌呤与元托普林对冬樱花 Withania somnifera 的体外再生、叶绿素和蛋白质含量影响的比较分析

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Saurabh Kumar, Shweta Singh, Madhuparna Banerjee
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引用次数: 0

摘要

偏东陵酸(mT)的使用严重影响了多种植物的组织培养结果。在这项研究中,对快速高效的薇甘菊微繁殖方案进行了标准化。此外,还比较了使用添加 mT 的 M.S. 培养基和添加不同浓度 B.A.P. + AdSO4 的 M.S. 培养基对体外再生的影响。补充了 I.B.A. 的 M.S. 培养基用于获得健康和有利的根系。对产生的小植株进行叶绿素和蛋白质总含量分析。比较结果表明,添加了 2.5 mg/l mT 的 M.S. 培养基效果更好,破芽率为 88.9 ± 0.42%,每个外植体的小芽倍增率为 22.4 ± 4.16,总叶绿素含量为 0.887 ± 0.004 mg/g,蛋白质含量为 25.67 ± 0.25%。在硬化过程中,最初用 mT 处理的植株有 80% 存活下来。而用添加了 1.0 毫克/升 B.A.P. + 50 毫克/升 AdSO4 的 M.S. 培养基处理后,芽的折断率为 61.1 ± 0.09%,每个外植体的嫩枝增殖率为 15.8 ± 7.81,总叶绿素含量为 0.7194 ± 0.0055 毫克/克,蛋白质含量为 23.33 ± 0.25%。在硬化过程中,最初用 B.A.P. + AdSO4 处理的小植株有 78% 存活下来。因此,研究得出结论,mT 可用作细胞分裂素的替代来源,以获得健康的小植株和更高的植株再生率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Comparative analysis of the effect of 6-benzylaminopurin versus meta-Topolin on in vitro regeneration, chlorophyll and protein contents in winter cherry Withania somnifera

Comparative analysis of the effect of 6-benzylaminopurin versus meta-Topolin on in vitro regeneration, chlorophyll and protein contents in winter cherry Withania somnifera

Use of meta-Topolin (mT) has significantly affected tissue culture results in several plant species. In this study, a fast and efficient micropropagation protocol for Withania somnifera was standardized. Also, a comparison was made between the effect of using M.S. media supplemented with mT and M.S. media supplemented with different concentrations of B.A.P. + AdSO4 on the in vitro regeneration of W. somnifera. M.S. media supplemented with I.B.A. was used to obtain a healthy and advantageous root system. The resulting plantlets were analyzed for their total chlorophyll and protein content. The comparison revealed that 2.5 mg/l mT supplemented M.S. media showed better results with 88.9 ± 0.42% bud breaking percentage, a shoot multiplication rate of 22.4 ± 4.16 shootlets per explants, total chlorophyll content of 0.887 ± 0.004 mg/g and protein content of 25.67 ± 0.25%. Healthy adventitious root systems were observed in regenerated shootlets inoculated in M.S. media supplemented with 2.0 mg/l I.B.A. During hardening, 80% of plants treated initially with mT survived. Whereas treatment with M.S. media supplemented with 1.0 mg/l B.A.P. + 50 mg/l AdSO4 resulted in bud breaking percentage of 61.1 ± 0.09%, a shoot multiplication rate of 15.8 ± 7.81 shootlets per explant, total chlorophyll content of 0.7194 ± 0.0055 mg/g and protein content of 23.33 ± 0.25%. During hardening, 78% of plantlets treated initially with B.A.P. + AdSO4 survived. Therefore, the study concludes that mT may be used as an alternative source of cytokinin for obtaining healthy plantlets with a higher rate of plant regeneration in W. somnifera.

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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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