{"title":"6-苄基氨基嘌呤与元托普林对冬樱花 Withania somnifera 的体外再生、叶绿素和蛋白质含量影响的比较分析","authors":"Saurabh Kumar, Shweta Singh, Madhuparna Banerjee","doi":"10.1007/s11240-024-02826-1","DOIUrl":null,"url":null,"abstract":"<p>Use of meta-Topolin (mT) has significantly affected tissue culture results in several plant species. In this study, a fast and efficient micropropagation protocol for <i>Withania somnifera</i> was standardized. Also, a comparison was made between the effect of using M.S. media supplemented with mT and M.S. media supplemented with different concentrations of B.A.P. + AdSO<sub>4</sub> on the in vitro regeneration of <i>W. somnifera</i>. M.S. media supplemented with I.B.A. was used to obtain a healthy and advantageous root system. The resulting plantlets were analyzed for their total chlorophyll and protein content. The comparison revealed that 2.5 mg/l mT supplemented M.S. media showed better results with 88.9 ± 0.42% bud breaking percentage, a shoot multiplication rate of 22.4 ± 4.16 shootlets per explants, total chlorophyll content of 0.887 ± 0.004 mg/g and protein content of 25.67 ± 0.25%. Healthy adventitious root systems were observed in regenerated shootlets inoculated in M.S. media supplemented with 2.0 mg/l I.B.A. During hardening, 80% of plants treated initially with mT survived. Whereas treatment with M.S. media supplemented with 1.0 mg/l B.A.P. + 50 mg/l AdSO<sub>4</sub> resulted in bud breaking percentage of 61.1 ± 0.09%, a shoot multiplication rate of 15.8 ± 7.81 shootlets per explant, total chlorophyll content of 0.7194 ± 0.0055 mg/g and protein content of 23.33 ± 0.25%. During hardening, 78% of plantlets treated initially with B.A.P. + AdSO<sub>4</sub> survived. Therefore, the study concludes that mT may be used as an alternative source of cytokinin for obtaining healthy plantlets with a higher rate of plant regeneration in <i>W. somnifera</i>.</p>","PeriodicalId":20219,"journal":{"name":"Plant Cell, Tissue and Organ Culture","volume":"129 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative analysis of the effect of 6-benzylaminopurin versus meta-Topolin on in vitro regeneration, chlorophyll and protein contents in winter cherry Withania somnifera\",\"authors\":\"Saurabh Kumar, Shweta Singh, Madhuparna Banerjee\",\"doi\":\"10.1007/s11240-024-02826-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Use of meta-Topolin (mT) has significantly affected tissue culture results in several plant species. In this study, a fast and efficient micropropagation protocol for <i>Withania somnifera</i> was standardized. Also, a comparison was made between the effect of using M.S. media supplemented with mT and M.S. media supplemented with different concentrations of B.A.P. + AdSO<sub>4</sub> on the in vitro regeneration of <i>W. somnifera</i>. M.S. media supplemented with I.B.A. was used to obtain a healthy and advantageous root system. The resulting plantlets were analyzed for their total chlorophyll and protein content. The comparison revealed that 2.5 mg/l mT supplemented M.S. media showed better results with 88.9 ± 0.42% bud breaking percentage, a shoot multiplication rate of 22.4 ± 4.16 shootlets per explants, total chlorophyll content of 0.887 ± 0.004 mg/g and protein content of 25.67 ± 0.25%. Healthy adventitious root systems were observed in regenerated shootlets inoculated in M.S. media supplemented with 2.0 mg/l I.B.A. During hardening, 80% of plants treated initially with mT survived. Whereas treatment with M.S. media supplemented with 1.0 mg/l B.A.P. + 50 mg/l AdSO<sub>4</sub> resulted in bud breaking percentage of 61.1 ± 0.09%, a shoot multiplication rate of 15.8 ± 7.81 shootlets per explant, total chlorophyll content of 0.7194 ± 0.0055 mg/g and protein content of 23.33 ± 0.25%. During hardening, 78% of plantlets treated initially with B.A.P. + AdSO<sub>4</sub> survived. Therefore, the study concludes that mT may be used as an alternative source of cytokinin for obtaining healthy plantlets with a higher rate of plant regeneration in <i>W. somnifera</i>.</p>\",\"PeriodicalId\":20219,\"journal\":{\"name\":\"Plant Cell, Tissue and Organ Culture\",\"volume\":\"129 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Cell, Tissue and Organ Culture\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11240-024-02826-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell, Tissue and Organ Culture","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11240-024-02826-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Comparative analysis of the effect of 6-benzylaminopurin versus meta-Topolin on in vitro regeneration, chlorophyll and protein contents in winter cherry Withania somnifera
Use of meta-Topolin (mT) has significantly affected tissue culture results in several plant species. In this study, a fast and efficient micropropagation protocol for Withania somnifera was standardized. Also, a comparison was made between the effect of using M.S. media supplemented with mT and M.S. media supplemented with different concentrations of B.A.P. + AdSO4 on the in vitro regeneration of W. somnifera. M.S. media supplemented with I.B.A. was used to obtain a healthy and advantageous root system. The resulting plantlets were analyzed for their total chlorophyll and protein content. The comparison revealed that 2.5 mg/l mT supplemented M.S. media showed better results with 88.9 ± 0.42% bud breaking percentage, a shoot multiplication rate of 22.4 ± 4.16 shootlets per explants, total chlorophyll content of 0.887 ± 0.004 mg/g and protein content of 25.67 ± 0.25%. Healthy adventitious root systems were observed in regenerated shootlets inoculated in M.S. media supplemented with 2.0 mg/l I.B.A. During hardening, 80% of plants treated initially with mT survived. Whereas treatment with M.S. media supplemented with 1.0 mg/l B.A.P. + 50 mg/l AdSO4 resulted in bud breaking percentage of 61.1 ± 0.09%, a shoot multiplication rate of 15.8 ± 7.81 shootlets per explant, total chlorophyll content of 0.7194 ± 0.0055 mg/g and protein content of 23.33 ± 0.25%. During hardening, 78% of plantlets treated initially with B.A.P. + AdSO4 survived. Therefore, the study concludes that mT may be used as an alternative source of cytokinin for obtaining healthy plantlets with a higher rate of plant regeneration in W. somnifera.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.