在藻酸盐凝胶中获得葡萄球菌单种和二元培养物的新方法，以及以儿茶酚胺为例研究活性化合物对这些培养物的作用

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI:10.1007/s10517-024-06217-6

S. V. Mart’yanov, A. V. Gannesen, V. K. Plakunov

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引用次数: 0

摘要

本研究提出了一种简单有效的方法，用于在模拟鼻腔自然微环境的藻酸钠凝胶基质中获得金黄色葡萄球菌和表皮葡萄球菌的单菌种和双菌种培养物。这些培养物用于研究去甲肾上腺素对金黄色葡萄球菌（侵袭性菌株）和表皮葡萄球菌（共生菌株）这两种细菌的单菌种和二元群落的影响。培养 24 小时后，金黄色葡萄球菌在二元群落中占优势，但随后被表皮葡萄球菌取代。较高浓度的去甲肾上腺素加速了这一过程，但并未对其产生根本改变。该模型可用于开发更有效的复合抗菌药物。

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A New Method for Obtaining Monospecies and Binary Cultures of Staphylococcus spp. in Alginate Gel and the Study of the Action of Active Compounds on These Cultures on the Example of Catecholamines

A simple and efficient method for obtaining monospecies and binary Staphylococcus aureus and Staphylococcus epidermidis cultures in sodium alginate gel matrix mimicking the natural microenvironment of the nasal cavity was proposed. The cultures were used for studying the effect of norepinephrine on monospecies and binary communities of two types of bacteria, S. aureus (invasive strain) and S. epidermis (commensal strain). After 24-h incubation, S. aureus predominated in the binary community, but later it was replaced by S. epidermis. Norepinephrine at higher concentrations accelerated this process without principally changing it. The model can be used to develop more effective complex antimicrobial drugs.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.