沙门氏菌噬菌体的分离与特征描述--噬菌体疗法治疗耐抗生素肠道感染的潜在药物

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2024-09-12 DOI:10.1007/s10517-024-06209-6

M. A. Pasivkina, M. N. Anurova, I. A. Kiseleva, A. A. Andreeva, A. M. Vorobev, T. E. Mizaeva, E. R. Mekhtiev, E. S. Zubkova, K. M. Alieva, A. R. Kuzmin, A. V. Karaulov, A. V. Aleshkin

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引用次数: 0

摘要

分离出了两种对沙门氏菌属致病菌株具有特异性活性的噬菌体。我们研究了噬菌体菌落的形态（大小、透明度、斑块边缘的形状和光晕）及其溶菌活性和与微生物细胞相互作用的范围（吸附率、潜伏期和繁殖效率）。通过全基因组测序，我们确定了噬菌体的分类位置，并验证了不存在编码毒素、粘附蛋白和入侵蛋白的多余基因，以及导致抗生素耐药性的致病岛。此外，我们还研究了噬菌体在不同物理条件下的稳定性及其生产率。

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Isolation and Characterization of Salmonella Bacteriophages as Potential Agents for Phage Therapy of Antibiotic-Resistant Intestinal Infections

Two bacteriophages specifically active against to pathogenic strains of the Salmonella genus were isolated. The morphology of phage colonies (size, transparency, and shape of the plaque edge, and halo) and the spectrum of their lytic activity and interaction with microbial cells (adsorption rate, duration of the latency, and reproductive efficiency) were examined. Using genome-wide sequencing, we determined the taxonomic position of bacteriophages and verified the absence of unwanted genes encoding toxins, adhesins, and invasins, as well as pathogenicity islands responsible for antibiotic resistance. In addition, phage stability under different physical conditions and their productivity were studied.

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.