罗勒粗提取物及其溶剂馏分的抗氧化活性和免疫增强活性研究

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-08-29 DOI:10.1177/1934578x241279050

Jingjing Gu, Xiaolan Ge, Mengjuan Zhang, Lili Wang, Wei Ma

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引用次数: 0

摘要

目的：罗勒具有多种生物活性，对其提取物进行充分研究有助于明确其具有生物活性的主要成分。方法：采用不同极性的溶剂萃取罗勒粗萃取物（CE）：采用不同极性的溶剂提取罗勒粗提取物（CE），并测定提取物中总多酚（TP）和总黄酮（TF）的含量。提取物中的化合物采用高效液相色谱-串联质谱法（LC-MS/MS）进行鉴定。评估了它们的抗氧化和免疫增强活性。结果表明获得了 CE 及其四种溶剂馏分，包括石油醚提取物（PE）、乙酸乙酯提取物（EE）、正丁醇提取物（BE）和水提取物（WE）。其中，BE 的 TP 和 TF 含量最高，其次是 EE，五种提取物均含有麦芽酚和熊果苷。BE 和 EE 具有较好的 1,1-二苯基-2-苦基肼（DPPH）自由基清除活性，而 TP 和 TF 含量较低的 CE 的 2，2'-氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除活性优于 BE 和 EE。此外，BE 和 EE 对免疫抑制小鼠相关指标的影响也优于其他提取物。结论罗勒提取物（BE 和 EE）的 TP 和 TF 含量较高，对免疫抑制小鼠的体外抗氧化活性和免疫增强效果较好。这为罗勒的应用提供了理论依据。

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Study on Antioxidant Activity and Immune-Enhanced Activity of Basil Crude Extract and its Solvent Fractions

Objective: Basil has various biological activities, and sufficient research on its extracts can help to clarify the main components that exert biological activity. Methods: Solvents with different polarities were used to extract the basil crude extract (CE), and the total polyphenol (TP) and total flavonoid (TF) contents of extracts were measured. The compounds of extracts were identified using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Their antioxidant and immune-enhanced activities were evaluated. Results: The CE and its four solvent fractions were obtained including petroleum ether extract (PE), ethyl acetate extract (EE), n-butanol extract (BE), and water extract (WE). Among them, BE had the highest TP and TF contents, followed by EE, and all five extracts contained maltol and arbutin. The BE and EE have better 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, while CE with less TP and TF contents showed better 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity than that of BE and EE. Besides, BE and EE also exert a better effect on relevant indicators of immunosuppressive mice than other extracts. Conclusion: The basil extracts (BE and EE), which have higher TP and TF contents, demonstrate better in vitro antioxidant activities and immune-enhancing effects on immunosuppressed mice. This provides a theoretical basis for the application of basil.

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.