探索从Trachyspermum roxburghianum (DC.) H. Wolff种子中提取的Siddhalepa Asamodagam精神的生物活性

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-08-21 DOI:10.1177/1934578x241271629

Dilan Jayawantha, Lankani Hettigoda, Tharindya Dinethri Mudalige, Priyani Ashoka Paranagama

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引用次数: 0

摘要

目的从罗布麻种子中提取的水蒸馏物历来被用于治疗胃部感染和胃溃疡。然而，目前还没有系统的研究来评估其功效。因此，本研究侧重于评估其潜在的健康益处，包括蒸馏物的化学成分。方法采用气相色谱-质谱法（GC-MS）对 Sid.AS 的化学成分进行鉴定和定量。采用 DPPH 和 ABTS 法对 Sid.AS 样品的抗氧化潜力进行体外评估。根据斯里兰卡阿育吠陀部规定的标准方法，对不同浓度的 Sid.AS 进行了抗菌、抗肥胖、抗糖尿病、抗炎和尿素酶抑制试验。与阳性对照组相比，Sid.AS 的 IC50 值较低，表明它具有明显的抗尿酸酶、抗炎、抗脂肪酶和抗氧化活性。这表明它在控制胃相关疾病、清除自由基以及通过抑制脂肪的分解和吸收来控制肥胖症方面具有潜力。此外，Sid.AS 还具有抑制α-淀粉酶和α-葡萄糖苷酶的作用，这表明它具有通过调节血糖水平来抗糖尿病的潜在活性。Sid.AS 对测试微生物具有很强的抗菌活性，抑制区更大，MIC 和 MLC 值更低，表明其在抗微生物感染方面具有很强的功效。结论 Sid.AS 的研究结果表明，它具有作为多功能生物活性草药蒸馏物的潜力，具有多种药理活性。我们的研究结果表明，Sid.AS 是一种前景广阔的天然草药提取物，具有多种药理特性，包括抗尿酸酶、抗氧化、抗炎、抗糖尿病、抗肥胖和抗菌活性。进一步的研究和开发可以探索其在各种治疗领域的潜在应用。

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Exploring the Bioactivity of Siddhalepa Asamodagam Spirit from Seeds of Trachyspermum roxburghianum (DC.) H. Wolff

ObjectivesThe water distillate derived from Trachyspermum roxburghianum seeds has been traditionally employed in medicine for treating stomach infections and gastric ulcers. However, no systematic study has been conducted to evaluate its efficacy. Therefore, the present study focused on evaluating the potential health benefits, including the chemical constituents of the distillate. This distillate was prepared and identified as Siddhalepa Asamodagam Spirit (Sid.AS).MethodsThe chemical constituents of Sid.AS were identified and quantified using Gas Chromatography-Mass Spectrometry (GC-MS) method. The antioxidant potential of Sid.AS samples was assessed in vitro using DPPH and ABTS assays. Various concentrations of Sid.AS were subjected to antimicrobial, anti-obesity, anti-diabetic, anti-inflammatory, and urease inhibition assays according to the standard methods specified by the Ayurvedic Department in Sri Lanka.ResultsThymol was identified as the major compound in Sid.AS through GC-MS analysis. Sid.AS demonstrated significant anti-urease, anti-inflammatory, anti-lipase, and antioxidant activities, as evidenced by low IC50 values compared to the positive controls. This suggests its potential in controlling gastric-related disorders, scavenging free radicals, and managing obesity by inhibiting the breakdown and absorption of fats. Additionally, Sid.AS exhibited inhibitory effects against alpha-amylase and alpha-glucosidase enzymes, indicating potential anti-diabetic activity by regulating blood sugar levels. Sid.AS displayed strong antimicrobial activity against tested microorganisms, with higher zones of inhibition and lower MIC and MLC values, indicating its effectiveness in combating microbial infections. Findings from the anti-lipase assay demonstrated activity comparable to that of the positive control, Orlistat.ConclusionThe findings of Sid.AS suggest its potential as a multi-functional bioactive herbal distillate with various pharmacological activities. Our results highlight that Sid.AS is a promising natural herbal extract with diverse pharmacological properties, including anti-urease, antioxidant, anti-inflammatory, anti-diabetic, anti-obesity, and antimicrobial activities. Further research and development could explore its potential applications in various therapeutic areas.

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.