绘制非特异性过氧化氢酶的选择性氧化图谱

Dominik Homann, Pascal Puellmann, Martin J. Weissenborn
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引用次数: 0

摘要

已经发现了几种非特异性过氧酶(UPOs),它们可以进行广泛的选择性氧官能化,因此是生物催化 "工具箱 "中的重要补充。为了使这些 "氧化工具 "得到广泛应用,为它们的底物偏好、化学选择性和区域选择性提供一份详细的 "用户手册 "至关重要。因此,我们选择了 16 种不同的底物和 15 种不同的 UPOs,并绘制了它们的偏好图。事实证明,各种 UPO 都具有高度选择性--根据底物的位置或化学特性进行区分--化学和区域选择性高达 99%,而周转次数 (TON) 则从几百次到数千次不等。这一 UPO 选择性图谱将成为新的化学酶途径的起点和蛋白质工程努力的起点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mapping Selective Oxidations of Unspecific Peroxygenases
Several unspecific peroxygenases (UPOs) have been identified that perform a broad range of selective oxyfunctionalizations and hence represent a pivotal addition to the biocatalysis 'toolbox'. To make these 'oxidation tools' broadly applicable it is crucial to provide a detailed 'user manual' for their substrate preference, chemo- and regioselectivity. We therefore selected 16 different substrates with a panel of 15 diverse UPOs and mapped their preferences. Various UPOs proved to be highly selective - discriminating based on either position or chemical properties of the substrate - with up to 99 % chemo- and regioselectivity while achieving turnover numbers (TONs) of a few hundred up to multiple thousands. This map of UPO selectivity shall serve as a starting point for new chemoenzymatic routes and starting points for protein engineering endeavors.
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