蔗糖和植物激素诱导的马铃薯块茎果肉绿原酸含量和代谢相关基因调控

IF 2.3 3区 农林科学 Q1 AGRONOMY
Huiqin Yang, Wei Luo, Dongli Gao
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引用次数: 0

摘要

绿原酸(5-O-咖啡酰奎宁酸,CGA)是马铃薯中含量最高的酚类化合物,是马铃薯抗虫和抗病的物质基础之一。然而,过量的 CGA 会影响马铃薯的口感。应用外源植物激素是通过转录调控途径的基因表达来调节 CGA 代谢的有效技术手段。在此,我们测定了施用蔗糖和多种植物激素时 CGA 的含量,并探讨了相关基因的表达变化。作为概念验证,研究了外源蔗糖和多种植物激素对马铃薯块茎果肉中 CGA 代谢的影响。经蔗糖处理的马铃薯块茎组织以及经脱落酸(ABA)、水杨酸(SA)和茉莉酸甲酯(MeJA)处理的块茎组织中的 CGA 含量明显降低。在这些处理中,SA的效果最为明显。与此同时,参与马铃薯 CGA 代谢的六个主要酶基因,包括苯丙氨酸氨解酶(PAL)、4-香豆酸辅酶 A 连接酶(4CL)、羟基肉桂酰辅酶 A(4CL)、苯丙氨酸氨解酶(PAL)、4-香豆酸辅酶 A 连接酶(4CL)和羟基肉桂酰辅酶 A(4CL)的表达发生了变化、分析了参与马铃薯 CGA 代谢的基因,包括苯丙氨酸氨基转移酶(PAL)、4-香豆酸辅酶 A 连接酶(4CL)、羟基肉桂酰 CoA 醌羟基肉桂酰转移酶(HQT)、羟基肉桂酰 CoA 莽草酸/醌羟基肉桂酰转移酶(HCT)、ρ-香豆酸 3'-羟化酶(C3H)和咖啡酰莽草酸酯酶(CSE)。在蔗糖处理下,HQT下调,而三个基因(PAL、4CL和CSE)明显上调,HCT和C3H的表达没有显著变化。ABA 可诱导 HCT 的表达,但抑制 PAL、4CL、HQT、C3H 和 CSE 的表达。在 SA 处理下,六种酶基因的表达均下调。MeJA 可诱导 HQT 的表达,但抑制 PAL、4CL、HCT、C3H 和 CSE 的表达。总之,蔗糖和植物激素可通过调节 CGA 途径相关基因的表达水平来调节马铃薯块茎果肉中 CGA 的积累。这些结果为进一步阐明CGA代谢的转录调控机制提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chlorogenic Acid Content and Metabolism-related Gene Regulation of Potato Tuber Flesh Induced by Sucrose and Phytohormones

Chlorogenic Acid Content and Metabolism-related Gene Regulation of Potato Tuber Flesh Induced by Sucrose and Phytohormones

Chlorogenic acid (5-O-caffeoylquinic acid, CGA) is the most abundant phenolic compound in potatoes, serving as one of the material bases for potato resistance to insects and diseases. Nevertheless, excessive CGA can impact the taste of potatoes. The application of exogenous phytohormones is an effective technical approach to regulate CGA metabolism through gene expression of transcriptional regulatory pathways. Herein, we determined the content of CGA under the application of sucrose and multiple phytohormones and explored the expression changes of related genes. As a proof-of-concept, the effects of exogenous sucrose and multiple phytohormones on CGA metabolism in potato tuber flesh were investigated. The CGA content was significantly decreased in sucrose-treated potato tuber tissues, as well as in those treated with abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA). Among these treatments, the effect of SA was the most obvious. Meanwhile, the expression changes of six major enzyme genes involved in potato CGA metabolism, including phenylalanine ammonia-lyase (PAL), 4-coumarate coenzyme A ligase (4CL), hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase (HQT), hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), ρ-coumarate 3’-hydroxilase (C3H), and caffeoyl shikimate esterase (CSE), were analyzed. Under sucrose treatment, HQT was down-regulated, whereas three genes (PAL, 4CL, and CSE) were notably up-regulated, and expressions of HCT and C3H did not show significant changes. ABA induced the expression of HCT, but inhibited the expression of PAL, 4CL, HQT, C3H, and CSE. The expression of all six enzyme genes was down-regulated under SA treatment. MeJA induced the expression of HQT, but inhibited the expression of PAL, 4CL, HCT, C3H, and CSE. Collectively, sucrose and phytohormones can regulate CGA accumulation in potato tuber flesh by regulating the expression levels of genes involved in the CGA pathway. These results provided a theoretical basis for further elucidating the transcriptional regulation mechanism of CGA metabolism.

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来源期刊
Potato Research
Potato Research AGRONOMY-
CiteScore
5.50
自引率
6.90%
发文量
66
审稿时长
>12 weeks
期刊介绍: Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as: Molecular sciences; Breeding; Physiology; Pathology; Nematology; Virology; Agronomy; Engineering and Utilization.
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