蓝光照射对赤霉酸信号转导和贝利葡萄树糖转运的影响

IF 3.5 3区 生物学 Q1 PLANT SCIENCES
Satoru Kondo, Miho Ishioka, Chihiro Hoshi, Hiroyuki Tomiyama, Yukino Masuda, Souma Murata, Takanori Saito, Katsuya Ohkawa, Hitoshi Ohara, Naoto Iwasaki, Sutthiwal Setha, Wei Heng, Jun Takeuchi, Yasushi Todoroki
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引用次数: 0

摘要

研究了蓝光照射对脱落酸(ABA)信号传导、糖代谢和转运以及光感受器和基因表达的影响,以阐明蓝光 LED 照射增加葡萄果实(Vitis labruscana L.)糖浓度的机制。与未经处理的对照组相比,蓝色发光二极管(LED)照射增加了喂食 13CO2 的葡萄果穗中 13C 光合成物的比例。经蓝色 LED 照射的浆果中果糖和葡萄糖的浓度以及 VvSWEET10、VvSUC11 和 VvSUS4 的表达量都有所增加。处理后 14 天,蓝光 LED 照射浆果的蔗糖浓度明显低于未处理的对照组。我们推测,经蓝色 LED 处理的浆果中蔗糖含量的降低与糖类最终将被导出转运体(VvSWEET10)、蔗糖转运体(VvSUC11)和蔗糖合成酶(VvSUS4)表达量的增加有关,并促进了 13C 光合成物从输入 13CO2 的叶片中的转运。蓝光照射增加了SNF1相关蛋白激酶(VvSnRK2.6)和ABA反应元件结合转录因子(VvABF1)的表达,同时降低了与ABA信号转导相关的蛋白磷酸酶2C9(VvPP2C9)基因的表达。蓝光照射增加了隐色素(VvCRYa)和趋光素(VvPHOT2)基因的表达,这两个基因是感光基因。施用吡拉菌素抗性样(PYL)-PP2C ABA 受体相互作用拮抗剂 AS6 不会影响葡萄果实中的内源 ABA 浓度,但会降低收获时的蔗糖浓度。施用 ABA 不会影响蔗糖、葡萄糖或果糖浓度,也不会影响 VvSnRK2.6 和 VvPP2C9 的表达。施用北氢愈创木脂酸(NDGA,ABA 生物合成过程中 9-顺式环氧类胡萝卜素二氧酶活性的抑制剂)对收获时的糖浓度没有影响。这些结果表明,感光基因表达的上调和 ABA 信号转导与葡萄浆果中的糖分浓度有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of blue-light irradiation on abscisic acid signaling and sugar translocation in Vitis labruscana L.H. Bailey grapevines

Effects of blue-light irradiation on abscisic acid signaling and sugar translocation in Vitis labruscana L.H. Bailey grapevines

The effects of blue-light irradiation on abscisic acid (ABA) signaling, sugar metabolism and translocation, and photoreceptors and gene expressions were investigated to clarify the mechanism by which blue-LED irradiation increases sugar concentrations in grape berries (Vitis labruscana L.). Blue light-emitting diode (LED) irradiation increased the portion of 13C-photosynthates in the grapevine clusters that were fed 13CO2; compared to the portion in the cluster in the untreated control. Fructose and glucose concentrations and the expressions of VvSWEET10, VvSUC11, and VvSUS4 in blue LED-irradiated berries were increased. The blue LED-irradiated berries’ sucrose concentrations were significantly lower than the untreated control at 14 days after treatment. We speculated that the blue LED-treated berries’ decreased sucrose was associated with the increased Sugars Will Eventually be Exported Transporter (VvSWEET10), sucrose transporter (VvSUC11), and sucrose synthase (VvSUS4) expressions and promoted the translocation of 13C-photosynthates from the leaves that were fed 13CO2. Blue-LED irradiation increased the expressions of SNF1-related protein kinases (VvSnRK2.6) and ABA responding element binding transcription factor (VvABF1), while decreasing the expression of protein phosphateses 2C9 (VvPP2C9) genes, which are related to ABA signaling. Blue-LED irradiation increased the expressions of cryptochrome (VvCRYa) and phototropin (VvPHOT2), which are photoreceptor genes. The application of the pyrabactin resistance-like (PYL)-PP2C ABA receptor interaction antagonist AS6 did not affect endogenous ABA concentrations in the grape berries, but it decreased sucrose concentrations at harvest. The application of ABA did not affect sucrose, glucose, or fructose concentrations or the expressions of VvSnRK2.6 and VvPP2C9. The application of nordihydroguaiaretic acid (NDGA, an inhibitor of 9-cis-epoxycarotenoid dioxygenase activity in ABA biosynthesis) did not affect sugar concentrations at harvest. These results suggest that upregulation of photoreceptor gene expressions and ABA signaling are associated with sugar concentrations in grape berries.

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来源期刊
Plant Growth Regulation
Plant Growth Regulation 生物-植物科学
CiteScore
6.90
自引率
9.50%
发文量
139
审稿时长
4.5 months
期刊介绍: Plant Growth Regulation is an international journal publishing original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research using hormonal, physiological, environmental, genetical, biophysical, developmental or molecular approaches to the study of plant growth regulation. Emphasis is placed on papers presenting the results of original research. Occasional reviews on important topics will also be welcome. All contributions must be in English.
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