Anna Kaczmarek , Anna Maciejewska , Katarzyna Kasperkiewicz , Magdalena Noszczyńska , Jolanta Łukasiewicz
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引用次数: 0
摘要
Asaia bogorensis 是一种革兰氏阴性细菌，从热带气候下生长的花朵和水果、蚊子的生殖系统中分离出来，很少从免疫力低下的病人身上分离出来。在欧洲，A. bogorensis 是造成矿泉水污染的罪魁祸首。脂多糖（LPS，内毒素）是细菌的重要表面抗原之一，也是细菌生物膜的要素之一。迄今为止，还没有关于 A. bogorensis LPS 结构的数据。化学分析和 1H、13C 核磁共振光谱发现了 A. bogorensis ATCC BAA-21 LPS 的 O 型特异多糖的新结构。结果表明，O 型抗原的重复单位是支链三糖，结构如下：→6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]-α-l-Rhap-(1→ .
Structure of the O-specific polysaccharide of Asaia bogorensis ATCC BAA-21 lipopolysaccharide
Asaia bogorensis is a Gram-negative bacterium isolated from flowers and fruits growing in tropical climate, reproductive system of mosquitoes, and rarely from immunocompromised patients. In Europe, A. bogorensis is responsible for the contamination of flavoured mineral waters. One of the important surface antigen and an element of the bacterial biofilm is lipopolysaccharide (LPS, endotoxin). To date, no data on A. bogorensis LPS structure has been reported. Chemical analysis and 1H,13C nuclear magnetic resonance spectroscopy revealed the novel structure of the O-specific polysaccharide of A. bogorensis ATCC BAA-21 LPS. It was concluded that the repeating unit of the O-antigen is a branched trisaccharide with the following structure: →6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]-α-l-Rhap-(1→ .
期刊介绍：
Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects.
Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence.
Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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