芪类衍生物使乳腺癌细胞对电离辐射敏感的功效

IF 2.5 Q3 CELL BIOLOGY
Dominika Komorowska, Agnieszka Zaczek, Sebastian Kalenik, Aleksandra Rodacka
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引用次数: 0

摘要

背景/目的:乳腺癌的治疗方法之一是手术切除肿瘤,并通过术后放疗预防复发。遗憾的是,由于癌细胞对电离辐射的敏感性较低,放疗并不总是足够有效。本研究旨在评估白藜芦醇、皮杉醇和多靛红对乳腺癌细胞的放射增敏特性:实验部分在三阴性乳腺癌细胞(HCC38)和激素依赖性细胞(MCF7)上进行。研究评估了细胞死亡水平、与细胞凋亡过程相关的基因(BAX、BCL-2)和蛋白质(CASPASE 3、8 和 P53)的表达变化、抗氧化酶(CATALASE、SOD、GPx1/2)和 NRF-2 的表达变化。此外,还评估了参与 DNA 修复过程的 RAD51 蛋白和组蛋白 H2AX 的表达水平。统计意义通过双向方差分析(ANOVA)和 Tukey 后检验(P < 0.05)进行评估:结果:电离辐射与白藜芦醇或皮萨单宁醇结合使用可通过内部和外部途径激活细胞凋亡过程。与 HCC38 细胞相比,MCF7 细胞对电离辐射和白藜芦醇的敏感性更高,这与细胞的抗氧化反应较弱和 DNA 损伤修复强度降低有关。电离辐射诱导的 DNA 修复在 HCC38 细胞中比在 MCF7 细胞中更有效:结论:白藜芦醇对两种品系的细胞都具有最高的放射增敏潜力。电离辐射与白藜芦醇(在较小程度上与皮夏单宁醇)结合对 MCF7 细胞的效果比对 HCC38 细胞更显著。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacy of Stilbene Derivatives in Sensitizing Breast Cancer Cells to Ionizing Radiation.

Background/aims: One of the treatments for breast cancer is surgical resection of the tumour and prevention of recurrence with postoperative radiotherapy. Unfortunately, radiotherapy is not always effective enough due to the low sensitivity of cancer cells to ionising radiation. This study aimed to evaluate the radiosensitising properties of resveratrol, piceatannol and polydatin on breast cancer cells, which differ in the presence of hormonal receptors on their surface.

Methods: The experimental part was carried out on triple-negative breast cancer cells (HCC38) and hormone-dependent cells (MCF7). The study assessed the level of cell death, changes in the expression of genes (BAX, BCL-2) and proteins related to the apoptosis process (CASPASE 3, 8 and P53), changes in the expression of antioxidant enzymes (CATALASE, SOD, GPx1/2) and NRF-2. Additionally, the expression level of RAD51 protein and histone H2AX, which are involved in DNA repair processes, was assessed. Statistical significance was evaluated by a two-way analysis of variance (ANOVA) followed by Tukey's post hoc test (p < 0.05).

Results: Ionising radiation in combination with resveratrol or piceatannol activates the apoptosis process by internal and external pathways. Greater sensitivity of MCF7 cells compared to HCC38 cells to ionising radiation in combination with resveratrol is associated with a weaker antioxidant response of cells and reduced intensity of DNA damage repair. DNA repair induced by ionising radiation occurs more effectively in HCC38 cells than in MCF7 cells.

Conclusion: Resveratrol has the highest radiosensitising potential among the tested stilbene for cells of both lines. The effectiveness of ionizing radiation in combination with resveratrol (to a lesser extent with piceatannol) is more significant in MCF7 than in HCC38 cells.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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